Cloning vectors comprising a restriction site bank and the const

Details Cloning vectors comprising a restriction site bank and the const Cloning vectors comprising a restriction site bank and the const

1984-05-11

1987-04-14

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

4351722, 435317, 435253, 935 22, 935 26, 935 27, 536 27, C12P 2100, C12N 1500, C12N 100, C12N 120

Patent

active

046578587

ABSTRACT:
Vectors are disclosed for cloning any foreign DNA in prokaryotic and eukaryotic organisms, which vectors comprise a restriction site bank containing a large number of unique restriction sites. The disclosed vectors permit the cloning of large DNA fragments and complete genes without the risk of unwanted DNA cleavage by restriction enzymes. Also disclosed are methods for preparing the aforementioned vectors and processes using the vectors for the cloning of DNA, for the production of polypeptides, and the like.

REFERENCES:
patent: 4528266 (1985-07-01), Pieczenik
Talmadge et al., 1980 "Construction of Plasmid Vectors with Unique Pst I Cloning Sites in a Signal Coding Region" Gene viz 235-241.
Rao et al., 1979 "Plasmid Pkc7: A vector Containing Ten Restriction Endonuclease Sites Suitable for Cloning DNA Segments" Gene v7 79-82.

Affiliated with

Also associated with

Rating

Cloning vectors comprising a restriction site bank and the const does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Cloning vectors comprising a restriction site bank and the const, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Cloning vectors comprising a restriction site bank and the const will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1785464