Cloning vector plasmid vector-primer derived therefrom and prepa

Details Cloning vector plasmid vector-primer derived therefrom and prepa Cloning vector plasmid vector-primer derived therefrom and prepa

1995-06-06

1997-04-29

Moody, Patricia R.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

4351723, 4353201, 935 18, 935 22, C12N 1564, C12N 1510, C12N 1585, C12N 1570

Patent

active

056248261

ABSTRACT:
Disclosed is a cloning vector plasmid suitable for preparing cDNA banks. The cloning vector plasmid of the present invention comprises, in the order mentioned from upstream to downstream region unless otherwise specified, a promoter PR1 acting in mammalian cells; an unique restriction enzyme site RE1 rarely existing in eukaryotic DNA and a promoter PR2 of RNA polymerase; an unique restriction enzyme site RE2 generating 3'-protruding dG tail; an unique arbitrary restriction enzyme site RE3; an unique arbitrary restriction enzyme site RE4 generating 3'-protruding terminus; an unique restriction enzyme site RE5 rarely existing in eukaryotic DNA and a promoter PR3 of RNA polymerase; and at least one kind of arbitrary restriction enzyme site RE6 generating 3'-protruding terminus existing at the arbitrary position before the restriction site RE1; and further, at arbitrary positions other than the above-described restriction enzyme sites and promoters, an origin OR1 for replication in mammalian cells, an origin OR2 for replication of a single-stranded phage, an origin OR3 for replication in E. coli, and a selective marker.

REFERENCES:
patent: 4843003 (1989-06-01), Henikoft et al.
patent: 5238834 (1993-08-01), Pruitt
Mead et al. 1986 Protein Engineering, 1;67-74.
Deininger 1987 Meth. Enzy. 152, 371-389.
Sargent et al 1983 Science 222, 135-139.
Okayama et al 1983 Molec. Cell. Bio 3, 280-289.
Pharmacia Catalog "Molecular Biologicals" 1986, 67-68.
Helfman et al (1983) PNAS 80, 31-35.
Palazzolo et al, Gene, vol. 52, pp. 197-206 (1987).
Pruitt, Gene, vol. 66, pp. 121-134 (1988).

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