Cloning vector, molecules of recombinant DNA, bacillus subtilis

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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435 711, 435 91, 435170, 4351721, 4351723, 43525231, 4353201, 435839, 536 27, 935 6, 935 8, 935 9, 935 22, 935 29, 935 33, 935 38, 935 59, 935 60, 935 61, 935 74, C12N 121, C12N 1509, C12N 1575

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050213407

ABSTRACT:
A description is given of a cloning vector useful for the expression and secretion of heterologous proteins in Bacillus subtilis, molecules of recombinant DNA formed by the said cloning vector, expediently cut at a restriction site located within the neutral protease gene, and by the sequence of heterologous DNA which codes for the protein of interest, Bacillus subtilis cells transformed with the said molecules and capable of expressing the sequence of heterologous DNA and of producing and secreting the protein coded by the said sequence of heterologous DNA in high yields.
The proteins obtained in this manner are useful in the fields of pharmaceuticals and foods, or as compounds for the production of substances which are normally obtained by chemical synthesis.

REFERENCES:
Ohmura et al. 1984, J. Biochem 95:87.
Yang et al. 1984, J. Bacteriol 160:15.
Uehara et al. 1979, J. Bacteriol 139:583.
Craig et al. 1982 Nature 295:345.
Yamazaki et al. 1983 J. Bacteriol 156: 327.

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