Cloning vector and a process for the preparation thereof

Details Cloning vector and a process for the preparation thereof Cloning vector and a process for the preparation thereof

1997-05-08

1999-11-16

Guzo, David

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 691, 435 914, 435 9141, 435 9142, 4352523, 4353201, 435440, C12Q 168, C12P 2102, C12N 1563, C12N 121

Patent

active

059855606

ABSTRACT:
This invention relates to a method for the preparation of a series of cloning vectors and such cloning vectors prepared therefrom. The method consists in the step of digestion of pRL1 derivative with a restriction enzyme BamHI and digesting the pIJ4026 by BglII and electroeluting the ermE gene. The linear DNA having pRL1 derivative is ligated with ermE gene and then transformed into E. coli GM2163. The transformants are screened for the presence of concatamers and that concatamers of pRL50 and pRL80 DNAs being isolated from E. coli. The transformants are selected under appropriate antibiotic selection pressure.

REFERENCES:
Lal et al. Construction of a hybrid plasmid capable of replication in Amycolatopsis mediterranei. Applied and Environmental Microbiology vol. 57 pp. 665-671, 1991.
Kumar et al. Efficient transformation of the cephamyin C producer Nocardia lactamdurans and development of shuttle and promoter-probe cloning vectors. Applied and Environmental Microbiology vol. 60 pp. 4086-4093, 1994.
Weinstock General Recombination is Escherichia coli, Chapter 60 in Escherichia coli and Salmonella typhimurium, Neidhardt Ed, Amer. Soc. for Microbiology, Washington D.C., 1987.

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