Cloning vector

Details Cloning vector Cloning vector

1978-02-27

1982-06-01

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Virus or bacteriophage, except for viral vector or...

435172, 435317, C12N 700, C12N 1500

Patent

active

043329012

ABSTRACT:
A cloning vector useful in recombining DNA (i.e. recombinant DNA studies) is made by mutagenesis of a satellite bacteriophage P4 wt or P4 vir.sub.1 and subsequent separation and purification of the mutant progeny in a cesium chloride equilibrium density gradient. On such a cesium chloride gradient the mutant is found in a range of densities from 1.35 to 1.42 g/ml at 24.degree. C. with peaks at about 1.42, 1.39, and 1.35 g/ml.

REFERENCES:
Lamanna et al., The Williams & Wilkins Co., pp. 723-727 (1965).
Metzler, Biochemistry, The Chemical Reactions of Living Cells, pp. 945-946.
Garland et al., Biochemistry of Genetic Engineering (1979).

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