Cloning vector

Details Cloning vector Cloning vector

1993-02-05

1994-12-27

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Vector, per se

4351723, C12N 1570

Patent

active

053765494

ABSTRACT:
A vector comprising a filamentous phage sequence containing a first copy of filamentous phage gene X and other sequences necessary for the phage to propagate is disclosed. The vector also contains a second copy of filamentous phage gene X downstream from a promoter capable of promoting transcription in a bacterial host. In a preferred form of the present invention, the filamentous phage is M13 and the vector additionally includes a restriction endonuclease site located in such a manner as to substantially inactivate the second gene X when a DNA sequence is inserted into the restriction site.

REFERENCES:
Studier et al. (1986) J. Mol. Biol. vol. 189:113-130.
Beck, et al., "Nucleotide Sequence And Genome Organization Of Filamentous Bacteriophages f1 and fd", Gene16:35-38, 1981.
Fitzgerald, et al., "Rapid Shotgun Cloning Utilizing The Two Base Recognition Endonuclease CviJI", Nucl. Acids Res., vol. 20, 14:3753-3762, 1992.
Pierce, et al., "A Positive Selection Vector For Cloning High Molecular Weight DNA By The Bacteriophage P1 System: Improved Cloning Efficacy", Proc. Natl. Acad. Sci. U.S.A., 89:2056-2060, 1992.
Fulford, et al., "Regulation Of Bacteriophage f1 DNA Replication", J. Mol. Biol., 203:49-62, 1988.
Waye, et al., "EcoK Selection Vectors For Shotgun Cloning Into M13 And Deletion Mutagenesis", Nucl. Acids. Res., vol. 13, 23:8561-8571, 1985.

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