Cloning the HinFI restriction and modification genes

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

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43525233, 4353201, 536 232, C12N 922, C12N 1555

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active

052159060

ABSTRACT:
Methods for cloning restriction enzymes and their corresponding modification enzymes by selecting clones resistant to in vitro digestion by the restriction enzyme and subsequent screening to identify those clones containing the restriction gene.

REFERENCES:
Kessler, C., et al. (1985) Gene 33, 1,51.
Walder, R. Y. et al. (1983) J. Biol. Chem. 258(2), 1235-1241.
Mann, M. B., et al. (1978) Gene 3, 97-112.

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