Chemistry: molecular biology and microbiology – Miscellaneous
Patent
1983-04-28
1987-03-10
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Miscellaneous
435 68, 4351723, 435253, 935 29, 935 72, 935 74, C12N 100, C12N 1500, C12P 2100
Patent
active
046491198
ABSTRACT:
The invention is a method for obtaining a chimeric plasmid which can serve as a vector for inserting one or more foreign or native genes into Corynebacterium and a second species of bacteria. The plasmid is the ligation product of plasmid DNA from Corynebacterium as well as plasmid DNA from a non-Corynebacterium species of bacteria. Also disclosed is a method for forming protoplasts from Corynebacterium organisms and transforming the protoplasts with vectors, e.g., the chimeric plasmids of the invention.
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patent: 4343906 (1982-08-01), Reusser et al.
patent: 4493893 (1985-01-01), Mielenz et al.
patent: 4508827 (1985-04-01), Olsen
patent: 4514502 (1985-04-01), Miwa et al.
Hopwood, D. A., Ann. Rev. Microbiol., 1981, vol. 35, pp. 237-272, "Genetic Studies with Bacterial Protoplasts".
White et al., Principles of Biochemistry, McGraw Hill NY, 1968, p. 105, 4th Edition.
Higashio Kanji
Rao Eswara A.
Shanabruch William G.
Sinskey Anthony J.
Walker Graham C.
Engellenner Thomas J.
Maslow James E.
Massachusetts Institute of Technology
Teskin Robin Lyn
Wiseman Thomas G.
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