Cloning system for Kluyveromyces species

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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435 68, 435 91, 435255, 4353171, 435320, 935 28, 935 37, 935 56, 536 27, C12N 1500, C12N 500, C12P 2100, C07H 1512

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048595969

ABSTRACT:
A new cloning system is described capable of expressing genetic material derived from recombinant DNA material, which comprises a yeast of the genus Kluyveromyces as a host. Suitable vectors are e.g. vectors containing autonomously replicating sequences (ARS) and vectors containing homologous Kluyveromyces DNA acting as a site for recombination with the host chromosome. New and preferred vectors are those containing ARS sequences originating from Kluyveromyces (KARS vectors). The genetically engineered new strains of Kluyveromyces produce, inter alia, lactase and chymosin.

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