Cloning, sequencing and expression of a comamonas...

Details Cloning, sequencing and expression of a comamonas... Cloning, sequencing and expression of a comamonas...

2007-05-08

2007-05-08

Saidha, Tekchand (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

C435S006120, C435S488000, C435S069100, C435S032000, C435S252330, C536S023200

Reexamination Certificate

active

10312585

ABSTRACT:
Cyclopentanone 1,2-monooxygenase (CPMO) fromComamonas(previouslyPseudomonas) sp. strain NCIMB 9872 carries out the second step of a degradation pathway that allows the bacterium to use cyclopentanol as a sole carbon source for growth. In the present invention there is reported the localization of the CPMO-encoding gene (cpnB) on a 4.3-kb SphI fragment, the determination of its sequence. The 550-amino acid CPMO polypeptide (Mt, 62,111) encoded by the gene was found to have 36.5% identity with the sequence of cyclohexanone 1,2-monooxygenase (CHMO) ofAcinetobactersp. strain NCIMB 9871. The 62-kDa CPMO was expressed inE. colias an IPTG-inducible protein.

REFERENCES:
patent: WO 02/06452 (2002-01-01), None

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