Cloning plasmid for streptomyces

Details Cloning plasmid for streptomyces Cloning plasmid for streptomyces

1981-06-22

1983-07-12

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435253, 435317, 435886, C12N 1500, C12N 120, C12N 100, C12R 1465

Patent

active

043931375

ABSTRACT:
Novel chemical compound plasmid pUC1061 obtained by deletion of .about.2.0 kilobases of DNA from the Streptomyces espinosus plasmid pUC6. This plasmid is useful as a cloning vehicle in recombinant DNA work. For example, using DNA methodology, a desired gene, for example, the glucose isomerase gene, can be inserted into the plasmid and the resulting plasmid can then be transformed into a suitable host microbe which, upon culturing, produces the desired glucose isomerase.

REFERENCES:
patent: 4273875 (1981-06-01), Manis
Science, 196, Apr., 1977, editorial.
Bibb, M. et al., Nature 284, 1980, 526-531.
Schottel, J. L. et al., J. Bacteriol., 146, (1981), 360-368.
Suarez, J. E. et al., Nature, 286, (1980), 527-529.
Thompson, C. J. et al., Nature, 286, (1980), 525-527.

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