Cloning of thermolabile glucoamylase gene into saccharomyces cer

Details Cloning of thermolabile glucoamylase gene into saccharomyces cer Cloning of thermolabile glucoamylase gene into saccharomyces cer

1985-09-16

1989-09-26

Teskin, Robin

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435 91, 435322, 435255, 435256, 435202, 435203, 435204, 435205, 536 27, 935 28, 935 60, 935 69, C12N 1500, C12N 120, C12N 500

Patent

active

048700142

ABSTRACT:
This inverntion concerns a glycoamylase gene cloned into the yeast Saccharomyces cerevisiae, method for cloning such a gene into such yeasts and cloning vehicles containing such a gene, suitable for use in Saccharomyces cerevisiae. Yeast containing a glucoamylase gene are of potential use in the brewing industry.

REFERENCES:
Yamashita I. and S. Fujui; Molecular Cloning of a Glucoamylase Producing Gene in the Yeast Saccharomycej; Agric. Biol. Chem. 47 (11), pp. 2689-2692, (1983).
Yamashita et al., Nucleotide Sequence of the Extracellular Glucoamylase Gene STAI in the Yeast S. diastaticus; J. Bacteriol. 161(2), pp. 567-573, (1985a).
Yamashita et al., Polymorphic Extracellular Glucoamylase Genes and their Evolutionary Origin in the Yeast S. diastaticus; J. Bacteriol. 161(2), pp. 574-582, (1985b).
Meaden P. et al., A DEX Gene Conferring Production of Extracellular Amyloglycosidase on Yeast; Gene 34, 325-334, (1985).
E. M. Southern, Detection of . . . Electrophoresis, J. Mol. Biol, (1975), 98, 503-507.
K. Nasmyth & S. Reid, Isolation of . . . cell-cycle gene, Proc. Natl. Acad. Sci. USA vol. 77, No. 4, 2119-2123, Apr. 1980.
J. D. Beggs, Transformation of . . . plasmid, Nature, vol. 275, Sep. 14, 1978, 104-109.
D. Cryer, R. Eccleshall, J. Marmur, Isolation of Yeast DNA, Chap. 3, Dept. of Biochemistry, Albert Einstein College of Medicine, New York, 39-44.
G. Godson, D. Vapnek, A Simple Method . . . Supercoiled DNA, Biochimica et Biophysics Acta, 299 (1973), 516-520.

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