Cloning of the biosynthetic pathway for chlortetracycline and te

Chemistry: molecular biology and microbiology – Vector – per se

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C12N 1500

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active

058664106

ABSTRACT:
The present invention describes a purified and isolated nucleic acid molecule which encodes for the biosynthetic pathway of tetracycline, chlortetracycline or an analogue thereof. The invention relates to the isolation and cloning of the nucleic acid molecule in an isolated fragment from Streptomyces aureofaciens and the expression of the biosynthetic gene in a heterologous host such as Streptomyces lividans.

REFERENCES:
patent: 4921801 (1990-05-01), Rao et al.
patent: 4935340 (1990-06-01), Baltz et al.
patent: 4992371 (1991-02-01), Wohllenben et al.
Old, R.W. and Primrose, S.B. Principles of Gene Manipulation. Blackwell Scientific Publications, Oxford, 1985.
Sambrooke et al. Molecular cloning. A laboratory Manual. pgs. 3.5 and 3.6 Cold Spring Harbor Laboratory Press 1989.
Reynes wt al. 1988 J. Gen Micro. 134 p. 585-598.
Butler et al. 1989 Mol. Gen Genet 215 p. 231-238.
Stanzak et al. Bio/Technology Vol. 4 p. 229-232.

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