Cloning of perilipin proteins

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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4351723, 4353201, 435325, 435363, 435366, 536 231, 536 235, C12N 1563, C12N 1509, C12N 510, C12N 1500

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060748427

ABSTRACT:
The present invention provides isolated nucleic acid sequences, i.e., polynucleotides, which encode a family of perilipin proteins. The present invention also provides isolated, substantially purified perilipin proteins which are useful as markers for differentiating true adipocytes from non-adipocyte cells which, as a result of pathophysiological conditions, assume adipocyte characteristics and become lipid-laden. The present invention further provides methods for producing a substantially purified perilipin protein and methods for detecting the presence of such perilipin proteins in a biological samples.

REFERENCES:
patent: 4675285 (1987-06-01), Clark et al.
patent: 5585462 (1996-12-01), Londos et al.
Kimmel et al. (Jan. 18-25, 1991) J. Cell. Biochem. Suppl 15B, p. 31, abstract CA309.
Greenberg et al. (May 1991) Clinical Research abstract.
Maruyama et al. (1986) Nuc. Acids Res 14: r151-r197.
Webster's II New Riverside University Dictionary, Soukhanov et al. (eds.) 1984, Houghton Mifflin Company, Boston, MA, p. 67.

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