Cloning of perilipin proteins

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues

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530359, 536 231, 536 235, 930 10, C07K 1447

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active

055854621

ABSTRACT:
The present invention provides isolated nucleic acid sequences, i.e., polynucleotides, which encode a family of perilipin proteins. The present invention also provides isolated, substantially purified perilipin proteins which are useful as markers for differentiating true adipocytes from non-adipocyte cells which, as a result of pathophysiological conditions, assume adipocyte characteristics and become lipid-laden. The present invention further provides methods for producing a substantially purified perilipin protein and methods for detecting the presence of such perilipin proteins in a biological samples.

REFERENCES:
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A. S. Greenberg et al., "Perilipin, A Lipid Droplet-Associated, Adipocyte Specific Protein; cDNA Cloning and Expression," 1991 Meeting (May 1991)--Abstract.
A. S. Greenberg et al., "Biochemistry and Hormonal Regulation of The Major Phosphoprotein Associated With The Lipid Storage Droplet In Adipocytes," Mid-Atlantic Lipid Clubs, Apr. 25-27, 1990--Abstract.
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Alan R. Kimmel et al., "cDNA Cloning and Expression of Perilipin, A Lipid Droplet-Associated, Adipocyte-Specific Protein," CA 309, J. of Cell. Biochem., Suppl. 15B, Jan. 18-25, 1991--Abstract, p. 31.
A. S. Greenberg et al., "Perilipin, A Major, Hormonally-Regulated, Adipocyte-Specific Phosphoprotein Associated With The Periphery of Lipid Storage Droplets," CA 306, J. of Cell. Biochem., Suppl. 15B, Jan. 18-25, 1991--Abstract, p. 30.
Constantine Londos et al., "Control of Isolated Adipocytes, Enzyme Trafficking, and The Surface of The Lipid Storage Droplet," CA 012, J. of Cell. Biochem., Suppl. 15B, Jan. 18-25, 1991--Abstract, p. 7.

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