Cloning of DNA encoding human motilin precursor and expression o

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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435 6, 435 91, 435 691, 435 697, 435 698, 4351723, 435320, 43525233, 536 27, 935 11, 935 13, 935 18, C12P 2100, C12P 1934, C12Q 168, C12N 1500

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049853562

ABSTRACT:
A cloned single-strand DNA comprising nucleotide sequence which encodes human motilin precursor, a cloned double-strand DNA consisting of the single-strand DNA and its complementary single-strand DNA, a fragment of the single- or double-strand DNA, a plasmid, in which the double-strand DNA or its fragment is integrated, as well as a process for the preparation of the single- or double-strand DNA.

REFERENCES:
Okayama and Berg, Mol. Cell. Biol., vol. 2, pp. 161-170, 1982.
Seino et al., FEBS. Lett., vol. 223(1), pp. 74-76, Oct. 1987, "Sequence of an Intestinal cDNA Encoding Human Motilin Precursor".
Poitras et al., Regulatory Peptides, vol. 5, pp. 197-208, 1983.
Suggs et al., Proc. Natl. Acad. Sci., vol. 178(11), pp. 6613-6617, Nov. 1981, "Use of Synthetic Oligonucleotides as Hybridization Probes: Isolation of Cloned cDNA Sequences for Humans for .beta..sub.2 -Microglobulin".
Ohtsuka et al., J. Biol. Chem., vol. 260, pp. 2605-2608, 1985.
Brown, J. C. et al., "Gastroenterology", vol. 6, pp. 401-404, (1974).
"Can. J. Biochem.", vol. 52, pp. 7-8, (1974), Schubert et al.
"Gastroenterology", vol. 80, pp. 456-460, (1981).

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