Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2005-08-02
2005-08-02
Wang, Andrew J. (Department: 1639)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C435S006120, C435S091100, C435S091400, C536S025300
Reexamination Certificate
active
06924112
ABSTRACT:
The present invention pertains to a process for isolating an intact clone of one target nucleic acid fragment having a known characteristic, from a group of fragments by preparing an initial library of clones from the group of fragments using a vector containing no more than a predetermined number of known restriction sites, preferably 1–3 restriction sites, subjecting the initial library to at least 10, and preferably between 50 and 70 restriction enzymes different from those to which the vector is susceptible, to produce a group of monodigested libraries, screening the group of monodigested libraries for the target fragment to determine those restriction enzymes to which the target fragment is insensitive, and subjecting the initial library to substantially all of the restriction enzymes to which the target fragment is insensitive, to produce a multidigested library having an intact clone of the target nucleic acid fragment. The target fragment can then be separated, transfected, reproduced, and studied or sequenced.
REFERENCES:
patent: 5252724 (1993-10-01), Kishimoto et al.
patent: WO 95/04745 (1995-02-01), None
Piug Deng and Jac A. Nickoloff, Site-Directed Mutagenesis Of Virtually Any Plasmid By Eliminating A Unique Site.
Jones, et al, Production Of A Vector To FAcilitate DNA Mutagenesis and Recombination, Biotech, vol. 16, No. 4, 1994.
Amersham Life Science Catalogue, pp. 164-165 XP002076172.
Biomethodes S.A.R.L.
Schnader Harrison Segal & Lewis LLP
Tran My-Chau T
Wang Andrew J.
LandOfFree
Cloning method by multiple-digestion, vectors for... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Cloning method by multiple-digestion, vectors for..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Cloning method by multiple-digestion, vectors for... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3494138