Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process...
Reexamination Certificate
2004-05-13
2008-07-08
Nashed, Nashaat T. (Department: 1656)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
C435S183000, C435S252300, C435S252350, C435S320100, C435S471000, C536S023200
Reexamination Certificate
active
07396660
ABSTRACT:
The present invention relates to the complete biosynthetic pathway for the formation of the LL-F28249 compounds and, most importantly, the major component LL-F28249α. The purified and isolated nucleic acid molecule encoding the proteins of the biosynthetic pathway, which is isolated from a wild-type or mutantStreptomyces, is fully described in FIG.6to FIG.6-39and SEQ ID NO:1. The DNA gene cluster and its expression in a suitable host enable the efficient production of the highly active natural metabolites and semisynthetic derivatives. The invention further concerns plasmids, vectors and host cells that contain and express the novel nucleic acid molecule. Of particular interest, the entire biosynthetic pathway fits compactly in three plasmids, Cos11, Cos36 and Cos40. The invention also concerns the purified and isolated biosynthesis proteins that are encoded by the whole DNA gene cluster. Additionally, the invention involves a new efficient, biochemical method of preparing moxidectin.
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Chaleff Deborah T.
Huang Chengjin
Ruppen Mark E.
Stephens Jerome
Moore William W.
Nashed Nashaat T.
Renda Barbara L.
Rosenblum Anne M.
Wyeth
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