Cloning by complementation and related processes

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues

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530300, C07K 500, C07K 1400

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active

059773057

ABSTRACT:
Disclosed are methods for detecting mammalian genes encoding proteins which can function in microorganisms, particularly yeast, to modify, complement, or suppress a genetic defect associated with an identifiable phenotypic alteration or characteristic in the microorganism. Disclosed also are mammalian DNA sequences cloned by the above method, as well as polypeptide products of the expression of the DNA sequences in procaryotic or eucaryotic host cells and antibody substances which are specifically immunoreactive with said expression products. More specifically, the present invention relates to methods for cloning mammalian genes which encode products which modify, complement or suppress a genetic defect in a biochemical pathway in which cAMP participates or in a biochemical pathway which is controlled, directly or indirectly, by a RAS-related protein, to products (RNA, proteins) encoded by the mammalian genes cloned in this manner, and to antibodies which can bind the encoded proteins.

REFERENCES:
Ammerer, G., "Expression of Genes in Yeast Using the ADCI Promoter," Meth. Enzymol., 101:192-201 (1983).
Barbacid, M., "ras Genes," Ann. Rev. Biochem., 56:779-827 (1987).
Beavo, "Multiple Phosphodiesterase Isozymes Background, Nomenclature and Implications", In: Cyclic Nucleotide Phosphodiesterases: Structure, Regulation and Drug Action, Chapter 1, Beavo, J. and Houslay, M.D., (eds.), John Wiley & Sons, New York. pp. 3-15 (1990).
Beavo et al., "Primary Sequence of Cyclic Nucleotide Phosphodiesterase Isozymes and the Design of Selective Inhibitors," TIPS (Reviews), 11:150-155 (1990).
Beavo, J.A., "Multiple Isozymes of Cyclic Nucleotide Phosphodiesterase," Advances in Second Messenger and Phosphoprotein Research, vol. II, Greengard et al., (eds.), Raven Press, New York, pp. 1-38 (1988).
Beckner et al., "The ras Oncogene Product p21 is not a Regulatory Component of Adenylate Cyclase," Nature, 317:71 (Sep. 5, 1985).
Bennetzen et al., "The Primary Stucture of the Saccharomyces cerevisiae Gene for Alcohol Dehydrogenase I," J. Biol. Chem., 257(6):3018-3025 (Mar. 25, 1982).
Biggin et al., "Buffer Gradient Gels and .sup.35 S Label as an Aid to Rapid DNA Sequence Determination," Proc. Nat'l Acad. Sci., USA, 80:3963-3965 (Jul., 1983).
Birchmeier et al., "RAS Proteins Can Induce Meiosis in Xenopus Oocytes," Cell, 43:615-621 (Dec., 1985).
Bradford, M.M., "A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding," Anal. Biochem. 72:248-254 (1976).
Brugge et al., "Expression of Rous Sarcoma Virus Transforming Protein pp60.sup.v-src in Saccharomyces cerevisiae Cells," Mol. & Cell. Biol., 7(6):2180-2187 (Jun., 1987).
Capon et al., "Activation of Ki-ras 2 Gene in Human Colon and Lung Carcinomas by Two Different Point Mutations," Nature, 304:507-513 (Aug., 1983).
Capon et al., "Complete Nucleotide Sequences of the T24 Human Bladder Carcinoma Oncogene and Its Normal Homologue," Nature, 302:33-37 (Mar. 3, 1983).
Charbonneau et al., "Identification of a Conserved Domain Among Cyclic Nucleotide Phosphodiesterases From Diverse Species," Proc. Natl. Acad. Sci., USA, 83:9308-9312 (Dec., 1986).
Chen et al., "Molecular Analysis of cDNA Clones and the Corresponding Genomic Coding Sequences of the Drosophila Dunce.sup.+ Gene, The Structural Gene for cAMP Phosphodiesterase," Proc. Nat'l Acad. Sci., USA, 83:9313-9317 (Dec., 1986).
Chirgwin et al., "Isolation of Biologically Active Ribonucleic Acid from Sources Enriched in Ribonuclease," Biochem., 18:5294-5299 (1979).
Colicelli et al., "Isolation and Characterization of a Mammalian Gene Encoding a High-Affinity cAMP Phosphodiesterase," Proc. Natl. Sci., USA, 86:3599-3603 (May, 1989).
Davis, "Molecular Genetics of the Cyclic Nucleotide Phosphodiesterases", In: Cyclic Nucleotide Phosphodiesterases: Structure, Regulation, and Drug Action, Beavo, J. and Houslay, M.D., Eds.; John Wiley & Sons, New York, pp. 227-241 (1990).
Davis et al., "A Partial Characterization of the Cyclic Nucleotide Phosphodiesterases of Drosophila melanogaster," Arch. Biochem. & Biophys. 203(1):412-421 (1980).
Davis et al., "A Simple Direct Assay of 3',5'-Cyclic Nucleotide Phosphodiesterase Activity Based on the Use of Polyacrylamide-Boronate Affinity Gel Chromatography," J. of Cyclic Nucleotide Research, 5(1):65-74 (1979).
Davis et al., "Cloning and Characterization of Mammalian Homologs of the Drosophila Dunce.sup.+ Gene," Proc. Nat'l. Acad. Sci., USA, 86:3604-3608 (May, 1989).
Davis et al., "Dunce Mutants of Drosophila melanogaster: Mutants Defective in the Cyclic AMP Phosphodiesterase Enzyme System," J. Cell. Biol., 90:101-107 (Jul., 1981).
De Vos et al., "Three-Dimensional Structure of an Oncogene Protein: Catalytic Domain of Human c-H-ras p21," Science, 239:888-893 (Feb., 1988).
DeFeo-Jones et al., "ras-Related Gene Sequences Identified and Isolated from Saccharomyces cerevisiae," Nature, 306:707-709 (Dec., 1983).
DeFeo-Jones et al., "Mammalian and Yeast ras Gene products: Biological Function in Their Heterologous Systems," Science, 228:179-184 (Apr. 12, 1985).
Dhar et al., "Nucleotide Sequence of Two ras.sup.H Related-Genes Isolated from the Yeast Saccharomyces cerevisiae," Nucl. Acids Res., 12:3611-3618 (1984)
Dudai, Y., "Neurogenetic Dissection of Learning and Short Term Memory in Drosophila," Ann. Rev. Neurosci., 11:537-563 (1988).
Eckmann et al., "Rolipram Major Depression: Results of a Double Blind Comparative Study With Amitriptyline," Current Therapeutic Research, 43(2):291-295 (Feb., 1988).
Ellis et al., "The p21 src Genes of Harvey and Kristen sarcoma viruses Originate from Divergent Members of a Family of Normal Vertebrate Genes," Nature, 292:506-511 (1981).
Faure et al., "Disruption of Dictyostelium discoideum Morphogenesis by Overproduction of cAMP Phosphodiesterase,"Proc. Nat'l Acad. Sci., USA, 85:8076-8080 (Nov., 1988).
Field et al., "Cloning and Characterization of CAP, the S. cerevisiae Gene Encoding the 70 kd Adenylyl Cyclase-Associated Protein," Cell, 61:319-327 (Apr. 20, 1990).
Field et al., "Mutations of the Adenylyl Cyclase Gene That Block RAS Function in Saccharomyces cerevisiae, " Science, 247:464-467 (Jan., 1990).
Field et al., "Purification of a RAS-Responsive Adenylyl Cyclase Complex from Saccharomyces cerevisiae by Use of an Epitope Addition Method," Mol. Cell. Biol., 8(5):2159-2165 (May, 1988).
Fukui et al., "Molecular Cloning and Sequence Analysis of a ras Gene from Schizosaccharomyces pombe," EMBO J., 4:687-691 (1985).
Fukui et al., "Role of a ras Homolog in the Life Cycle of Schizosaccharomyces pombe," Cell, 44:329-336 (Jan. 31, 1986).
Gerst et al., "CAP Is a Bifunctional Component of the Saccharomyces cerevisiae Adenylyl Cyclase Complex," Mol. & Cell Biol., 11(3):1248-1257 (Mar., 1991).
Goldner et al., "Eine Neue Xanthin-Synthese," Ann. Chem. 691:142-158 (1966).
Hanahan, D., "Studies on Transformation of Escherichia coli with Plasmids," J. Mol. Biol., 166:557-580 (1983).
Hancock et al., "All ras Proteins Are Polyiosprenylated but Only Some Are Palmitoylated," Cell, 57:1167-1177 (Jun., 1989).
Henikoff et al., "Isolation of a Gene from Drosophila by Complementation in Yeast," Nature, 289:33-37 (Jan., 1981).
Ito et al., "Transformation of Intact Yeast Cells Treated with Alkali Cations," J. Bacteriol., 153:163-168 (Jan., 1983).
Kataoka et al., "DNA Sequence and Characterization of the S. cerevisiae Gene Encoding Adenylate Cyclase," Cell, 43:493-505 (Dec., 1985).
Kataoka et al., "Functional Homology of Mammalian and Yeast RAS Genes," Cell, 40:19-26 (Jan., 1985).
Kataoka et al., "Genetic Analysis of Yeast RAS1 and RAS2 Genes," Cell, 37:437-445 (Jun., 1984).
Kitayama et al., "A ras-Related Gene with Transformation Suppressor Activity," Cell 56:77-84 (Jan., 1989).
Le Trong et al., "Amino Acid Sequence of the Cyclic GMP Stimulated Cyclic Nucleotide Phosphodiesterase from Bovine Heart," Biochemistry, 29:10280-10288 (1990).
Lee & Nurse, "Complementation Used to Clone a Human Homologue of the Fission Yeast Cell Cycle Control Gene cdc2," Nature, 327:31-35 (1987).
Livi et al., "Cloning and Expressi

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