Cloning and sequencing of the alcohol dehydrogenase II gene from

Details Cloning and sequencing of the alcohol dehydrogenase II gene from Cloning and sequencing of the alcohol dehydrogenase II gene from

1988-05-31

1992-11-10

Kepplinger, Esther L.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

435 6, 435190, 435 26, 935 9, 935 60, C12N 1512

Patent

active

051625164

ABSTRACT:
The alcohol dehydrogenase II gene from Zymomonas mobilis has been cloned and sequenced. This gene can be expressed at high levels in other organisms to produce acetaldehyde or to convert acetaldehyde to ethanol.

REFERENCES:
patent: 5000000 (1991-03-01), Ingram et al.
T. Conway, G. W. Sewell, Y. A. Osman, and L. O. Ingram, k"Cloning and Sequencing of the Alcohol Dehydrogenase II Gene from Zymomonas mobilis" J. Bacteriol. 169(6) 1987 pp. 2591-2597.
Conway, T., Y. A. Osman, J. I. Konnan, E. M. Hoffmann, and L. O. Ingram (1987) "Promoter and nucleotide sequences of the Zymomonas mobilis pyruvate decarboxylase," J. Bacteriol. 169:949-954.
Hoppner, T. C., and H. W. Doelle (1983) "Purification and kinetic characteristics of pyruvate decarboxylase and ethanol dehydrogenase from Zymomonas mobilis in relation to ethanol production," Eur. J. Appl. Microbiol. Biotechnol. 17:152-157.
Kinoshita, S., T. Kakizono, K. Kadota, K. Das, and H. Taguchi (1985) "Purification of two alcohol dehydrogenases from Zymomonas mobilis and their properties," Appl. Microbiol, Biotechnol. 22:249-254.
Montenecourt, B. S. (1985) "Zymomonas, a unique genus of bacteria," pp. 261-289 in A. L. Demain and N. A. Solomon (eds.), Biology of industrial microorganisms, Benjamin-Cummings Publishing Co., Menlo Park, CA.
Neale, A. D., R. K. Scopes, J. M. Kelly, and R. E. H. Wettenhall (1986) "The two alcohol dehydrogenases of Zymomonas mobilis: purification by differential dye ligand chromatography, molecular characterization and physiological role," Eur. J. Biochem. 154:119-124.
Scopes, R. K. (1983) "An iron-activated alcohol dehydrogenase," FEBS Lett. 156:303-306.
Wills, C., P. Kratofil, D. Londo, and T. Martin (1981) "Characterization of the two alcohol dehydrogenases of Zymomonas mobilis," Arch. Biochem. Biophys. 210:775-785.

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