Cloning and sequencing of allergens of dermatophagoides

Details Cloning and sequencing of allergens of dermatophagoides Cloning and sequencing of allergens of dermatophagoides

1993-12-29

2004-02-10

Housel, James (Department: 1648)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C435S069300, C435S068100, C435S069100, C435S069700, C435S069800, C435S071100, C435S173300, C435S252300, C435S320100, C514S002600, C536S023100, C536S023400

Reexamination Certificate

active

06689876

ABSTRACT:

FUNDING
Work described herein was funded by grants from the Princess Margaret Children's Medical Research Foundation, the Australian Health and Medical Research Council and the Asthma Foundation of Australia.
BACKGROUND
Recent reports have documented the importance of responses to the Group I and Group II allergens in house dust mite allergy. For example, it has been documented that over 60% of patients have at least 50% of their anti-mite antibodies directed towards these proteins (Lind, P. et al.,
Allergy
, 39:259-274 (1984); van der Zee, J. S. et al.,
J. Allergy Clin. Immunol
., 81:884-896 (1988)). It is possible that children show a greater degree of reactivity (Thompson, P. J. et al.,
Immunology
, 64:311-314 (1988)). Allergy to mites of the genus Dermatophagoides (D.) is associated with conditions such as asthma, rhinitis and ectopic dermatitis. Two species,
D. pteronyssinus
and
D. farinae
, predominate and, as a result, considerable effort has been expended in trying to identify the allergens produced by these two species.
D. pteronyssinus
mites are the most common Dermatophagoides species in house dust in Western Europe and Australia. The species
D. farinae
predominates in other countries, such as, North America and Japan (Wharton, G. W.,
J. Medical Entom
, 12:577-621 (1976)). It has long been recognized that allergy to mites of this genus is associated with diseases such as asthma, rhinitis and atopic dermatitis. It is still not clear what allergens produced by these mites are responsible for the allergic response and associated conditions.
SUMMARY OF THE INVENTION
The present invention relates to isolated DNA which encodes a protein allergen of Dermatophagoides (D.) house dust mite) or a peptide which includes at least one epitope of a protein allergen of a house dust mite of the genus Dermatophagoides. It particularly relates to DNA encoding major allergens of the species
D. farinae
, designated
Der f
I and
Der f
II, or portions of these major allergens (i.e., peptides which include at least one epitope of
Der f
I or of
Der f
II). It also particularly relates to DNA encoding major allergens of
D. pteronyssinus
, designated
Der p
I and
Der p
II, or portions of these major allergens (i.e., peptides which include at least one epitope of
Der p
I or of
Der p
II.
The present invention further relates to proteins and peptides encoded by the isolated Dermatophagoides (e.g.,
D. farinae, D. pteronyssinus
) DNA. Peptides of the present invention include at least one epitope of a
D. farinae
allergen (e.g., at least one epitope of
Der f
I or of
Der f
II) or at least one epitope of a
D. pteronyssinus
allergen (e.g., at least one epitope of
Der p
I or of
Der p
II). It also relates to antibodies specific for
D. farinae
proteins or peptides and to antibodies specific for
D. pteronyssinus
proteins or peptides.
Dermatophagoides DNA, proteins and peptides of the present invention are useful for diagnostic and therapeutic purposes. For example, isolated
D. farinae
protein or peptide can be used to detect sensitivity in an individual to house dust mites and can be used to treat sensitivity (reduce sensitivity or desensitize) in an individual, to whom therapeutically effective quantities of the
D. farinae
protein or peptide is administered. For example, isolated
D. farinae
protein allergen, such as
Der f
I or
Der f
II can be administered periodically, using standard techniques, to an individual in order to desensitize the individual. Alternatively, a peptide which includes at least one epitope of
Der f
I or of
Der f
II can be administered for this purpose. Isolated
D. pteronyssinus
protein allergen, such as
Der p
I or
Der p
II, can be administered as described for
Der f
I or
Der f
II. Similarly a peptide which includes at least one
Der p
I epitope or at least one
Der p
II epitope can be administered for this purpose. A combination of these proteins or peptides (e.g.,
Der f
I and
Der f
II;
Der p
I and
Der p
II; or a mixture of both
Der f
and
Der p
proteins) can also be administered. The use of such isolated proteins or peptides provides a means of desensitizing individuals to important house dust mite allergens.


REFERENCES:
patent: B 3132771 (1973-01-01), None
patent: WO88 10297 (1988-12-01), None
Chua et al.,Int. Arch. Allergy Appl. Immunol., 91:118-123, 1990.
Greene et al.,Int. Arch. Allergy Appl. Immunol., 92:30-38, 1990.
Griffin et al.,J. Allergy Clin. Immunol., 84:108-117, 1989.
Heymann et al.,J. Allergy Clin. Immunol., 83:1055-1067, 1989.
Heymann et al.,Journal of Immunology, 137:2841-2847, 1986.
Krillis et al.,J. Allergy&Clin. Immunology, 74:142-146, 1984.
Lamb et al.,Allergy, 19:389-393, 1989.
Lamb et al.,Journal of Immunological Methods, 110:1-10, 1988.
O'Hehir et al.,Int. Arch Allergy Appl. Immunol., 88:170-172, 1989.
Pierce et al.,Biochem Genetics, 109:191, Abstract No. 143/03, 1986.
Stewart et al.,Int. Archs. Allergy Appl. Immunol., 83:384-359, 1987.
Asian Pacific Journal of Allergy&Immunology, 4(1):71, 1986, Abstract.
Stewart et al.,Proceedings of the DPC 1st International Symposium on Allergy&Molecular Biology, Apr. 11-12, 1988, Laguana Niguel, California, USA.
Stewart et al.,Workshop XIV Congress European Acad. Allergy and Clinical Immunology, Berlin, Germany, Sep. 1989.
Stewart et al.,Int. Archs. Allergy Appl. Immunology, 83:44-51, 1987.
Stewart et al.,Int. Arch. Allergy Appl. Immunology, 69:224-230, 1982.
Thomas et al.,Biochem. Genetics, Abstract No. 72092, 113:179, 1990.
Thomas et al.,Int. Archs. Appl. Immunol., 85:127-129, 1988.
Thomas et al., Workshop XIV Congress European Acad. Allergy in Clinical Immunology, Boston, MA, USA, Sep., 1989.
Tovey et al.,J. Exp. Medicine, 170:1457-1462, 1989.
Ford et al.,Clinical and Experimental Allergy, 20:27-31, 1989.
Yasueda et al.,Chemical Abstracts, 105:552, Abstract No. 189004, 1986.
Yuuki et al.,Japan J. Allergol., 39(6):557-561, 1990.
Chua et al.,Chemical Abstracts, 108:148, Abstract No. 217095.
Thomas et al., The UCB Institute of Allergy, Mite Allergy a World-Wide Problem, Bad Kreuznach, Sep. 1-2, 1987.
Chapman et al., The UCB Institute of Allergy, Mite Allergy a World-Wide Problem, Bad Kreuznach, Sep. 1-2, 1987.
Chua et al.,Journal of Experimental Medicine, 167(1):175-182, 1988.

Affiliated with

Also associated with

Rating

Cloning and sequencing of allergens of dermatophagoides does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Cloning and sequencing of allergens of dermatophagoides, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Cloning and sequencing of allergens of dermatophagoides will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3350711