Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Reexamination Certificate
1999-04-20
2002-07-23
Scheiner, Laurie (Department: 1648)
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
C536S023100, C536S023200, C422S184100, C422S185000, C422S275000, C435S069300, C435S252300, C435S252330, C435S254200
Reexamination Certificate
active
06423837
ABSTRACT:
FUNDING
Work described herein was funded by grants from the Princess Margaret Children's Medical Research Foundation, the Australian Health and Medical Research Council and the Asthma Foundation of Australia.
BACKGROUND
Recent reports have documented the importance of responses to the Group I and Group II allergens in house dust mite allergy. For example, it has been documented that over 60% of patients have at least 50% of their anti-mite antibodies directed towards these proteins (Lind, P. et al.,
Allergy
, 39:259-274 (1984); van der Zee, J. S. et al.,
J. Allergy Clin. Immunol
., 81:884-896 (1988)). It is possible that children show a greater degree of reactivity (Thompson, P. J. et al,
Immunology
, 64:311-314 (1988)). Allergy to mites of the genus Dermatophagoides (D.) is associated with conditions such as asthma, rhinitis and ectopic dermatitis. Two species,
D. pteronyssinus
and
D. farinae
, predominate and, as a result, considerable effort has been expended in trying to identify the allergens produced by these two species.
D. pteronyssinus
mites are the most common Dermatophagoides species in house dust in Western Europe and Australia. The species
D. farinae
predominates in other countries, such as, North America and Japan (Wharton, G. W.,
J. Medical Entom
, 12:577-621 (1976)). It has long been recognized that allergy to mites of this genus is associated with diseases such as asthma, rhinitis and atopic dermatitis. It is still not clear what allergens produced by these mites are responsible for the allergic response and associated conditions.
SUMMARY OF THE INVENTION
The present invention relates to isolated DNA which encodes a protein allergen of Dermatophagoides (D.) house dust mite) or a peptide which includes at least one epitope of a protein allergen of a house dust mite of the genus Dermatophagoides. It particularly relates to DNA encoding major allergens of the species
D. farinae
, designated
Der f
I and
Der f
II, or portions of these major allergens (i.e., peptides which include at least one epitope of
Der f
I or of
Der f
II). It also particularly relates to DNA encoding major allergens of
D. pteronyssinus
, designated
Der p
I and
Der p
II, or portions of these major allergens (i.e., peptides which include at least one epitope of
Der p
I or of
Der p
II.
The present invention further relates to proteins and peptides encoded by the isolated Dermatophagoides (e.g.,
D. farinae, D. pteronyssinus
) DNA. Peptides of the present invention include at least one epitope of a
D. farinae
allergen (e.g., at least one epitope of
Der f
I or of
Der f
II) or at least one epitope of a
D. pteronyssinus
allergen (e.g., at least one epitope of
Der p
I or of
Der p
II). It also relates to antibodies specific for
D. farinae
proteins or peptides and to antibodies specific for
D. pteronyssinus
proteins or peptides.
Dermatophagoides DNA, proteins and peptides of the present invention are useful for diagnostic and therapeutic purposes. For example, isolated
D. farinae
protein or peptide can be used to detect sensitivity in an individual to house dust mites and can be used to treat sensitivity (reduce sensitivity or desensitize) in an individual, to whom therapeutically effective quantities of the
D farinae
protein or peptide is administered. For example, isolated
D. farinae
protein allergen, such as
Der f
I or
Der f
II can be administered periodically, using standard techniques, to an individual in order to desensitize the individual. Alternatively, a peptide which includes at least one epitope of
Der f
I or of
Der f
II can be administered for this purpose. Isolated
D. pteronyssinus
protein allergen, such as
Der p
I or
Der p
II, can be administered as described for
Der f
I or
Der f
II. Similarly a peptide which includes at least one
Der p
I epitope or at least one
Der p
II epitope can be administered for this purpose. A combination of these proteins or peptides (e.g.,
Der f
I and
Der f
II;
Der p
I and
Der p
II; or a mixture of both
Der f
and
Der p
proteins) can also be administered. The use of such isolated proteins or peptides provides a means of desensitizing individuals to important house dust mite allergens.
REFERENCES:
patent: 5798099 (1998-08-01), Yuuki et al.
patent: 5876722 (1999-03-01), Yuuki et al.
patent: 3132771 (1973-01-01), None
patent: WO 88/10297 (1988-12-01), None
Chapman et al. (1987) “Epitope Mapping of Major Dust Mite (dermatophagoides) Allergens Using Monoclonal Antibodies”Mite Allergy:27-29.
Chua et al. (1990) “Expression of Dermatophagoides Pteronyssinus Allergen, Der p II, in Escherichia Coli and the Binding Studies with Human IgF”Chemical Abstracts113: 434 (Abstract No. 4264).
Chua et al. (1990) “Isolation of cDNA Coding for the Major Mite Allergen Der p II by IgE Plaque Immunoassay”Int. Arch. Allergy Appl. Immun. 91: 118-123.
Chua et al. “Sequence Analysis to cDNA Coding for a major Proteases”Chemical Abstracts108(217095): 148.
Ford et al. (1989) “The Spectrum of Low Molecular Weight House Dust Mite (Dermatophagoides Pteronyssinus) Allergens with Emphasis on Der p II”Clinical and Experimental Allergy20: 27-31.
Greene et al. (1990) “Antigenic Analysis of Group I House Dust Mite Allergens Using Random Fragments of Der p I Expresed by Recombant DNA Libraries”Int. Arch. Allergy Appl. Immun.92: 30-38.
Griffin et al. (1989) “Allergenic and Antigenic Relationship Between Three Species of Storage Mite and the House Dust Mite, Dermatophagoides Pteronyssinus”J. Allergy Clin. Immun.84:108-117.
Heyman et al. (1986) “Antigen Der f I from the Dust Mite Dermatophagoides Farinae: Structural Comparison with Der p I from Dermatophagoides Pteronyssinus and Epitope Specificity of Murine IgG and Human IgE Antibodies”Journal of Immunology137: 2841-2847.
Heyman et al. (1989) “Antigenic and Structural Analysis of Group II Allergens (Der f II and Der p II) from House Dust Mites (Dermatophagoides spp)”J. Allergy Clin. Immun.83: 1055-1067.
Krilis et al. (1984) “Antigens and Allergens from the Common House Dust Mite Dermatophagoides Pteronyssinus”J Allergy&Clin Immun.74: 142-6.
Lamb et al. (1989) “HLA Class II Restriction Specificity of Dermatophagoides spp. Reactive T Lymphocyte Clones that Support IgE Syntesis”Clinical and Exper. Allergy19: 389-393.
Lamb et al. (1988) “The Use of Nitrocellulose Immunoblots for the Analysis of Antigen Recognition by T Lymphocytes”Journal of Immunological Methods110: 1-10.
O'Hehir et al. (1986) “Colonal Analysis of the Cellular Immune Response to the House Dust Mite Cematophagoides Farinae”Int. Arch. Allergy Appl. Immun.88: 170-172.
Pierce et al. (1986) “Molecular Cloning ofSchistosama masoniAllergens”Biochemical Genetics109: 191 (Abstract No. 143703).
Stewart (1982) “Isolation and Characterization of the Allergen Dpt 12 fromDermatophagoides pteronyssinusby Chromatofocusing”Int. Arch. Allergy Appl. Immun.69: 224-230.
Stewart and Holt (1987) “Immunogenicity and Tolerogenicity of a Major House Dust Mite Allergen, der p I fromDermatophasgoides pteronyssinus, in Mice and Rats”Int. Archs. Allergy Appl. Immun.83(1): 44-51.
Stewart et al. (1988) “An Allergen & Antigenic Mapping Analysis of a Major Mite Allergen, Der p I” Proceedings of the DPC 1st International Symposium on Allergy & Molecular Biology,Advances in Biosciences: Allergy and Molecular Biology74: 297-310.
Stewart et al. (1989) “Epitope Mapping Analysis of the Major Mite Allergens Using Synthetic Peptides”Proceedings of the Workshop of the XIV Congress of the European Academy of Allergy and Clinical Immunology, Berlin.
Stewart et al. (1987) “In vitro Translation of Messenger RNA from House Dust Mite Dermatophagoides Pteronyssinus”Int. Archs. Allergy Appl. Immun.83: 384-359.
Stewart et al. (1986) “The Physiochemical Characterization of a Major Protein Allergen from the House Dust Mite,Dermatophagoides pteronyssinus”Asian Pacific J. of Allergy&Immun.4(1):71.
Thomas et al. (1990) “Analysis and Expression of cDNA Clones Coding for House Dust Mite Allergens”Biochem. Genetics113: 179 (Abstract No. 72092f).
Thomas et
Chua Kaw-Yan
Thomas Wayne Robert
DiGiorgio, Esq. Jeanne M.
Immulogic Pharmaceutical Corp.
Lahive & Cockfield LLP
Remillard, Esq. Jane E.
Scheiner Laurie
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