Cloning and expression of yeast STE13 and Dpp2 genes encoding di

Details Cloning and expression of yeast STE13 and Dpp2 genes encoding di Cloning and expression of yeast STE13 and Dpp2 genes encoding di

1984-02-29

1990-12-11

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

4351723, 435224, 435255, 435320, 536 27, 935 14, 935 28, 935 60, 935 69, 935 70, C12N 948, C12N 1500, C12N 120, C07H 1700

Patent

active

049770853

ABSTRACT:
A method is provided for expressing the proteases dipeptidyl aminopeptidases A and B which are useful for processing precursor proteins. Genes controlling these proteases may be insdrted into appropriate vectors and transformed into cultures. The proteases may either be utilized to process precursor proteins in vivo or may be extracted from cultures and used to process precursor proteins in vitro.

REFERENCES:
Ratzkin, B. et al., Proc. Natl. Acad. Sci., vol. 74, pp. 487-491, Feb., 1977.
Julius, D. et al., Cell, vol. 32, pp. 839-852, Mar., 1983.
Glover, D., Genetic Engineering Cloning DNA, pp. 66-78, Chapman and Hall, London & New York, 1980.
Jones, E. W. in Yeast Geneticss (Eds., Spencer, Spencer & Smith), Springer Verlag, N.Y., pp. 167, 180-185, 1983.

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