Cloning and expression of transforming growth factor .beta.2

Details Cloning and expression of transforming growth factor .beta.2 Cloning and expression of transforming growth factor .beta.2

1989-12-05

1993-06-22

Ellis, Joan

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

455 691, 455 695, 455 91, 4551723, 4552351, 4552402, 530350, 935 19, 935 32, 935 34, 935 47, 935 57, 935 62, 935 70, C12P 2102, C12P 1934, C12N 1500, C12N 700, C12N 500, C07K 300

Patent

active

052216209

ABSTRACT:
cDNA clones coding for TGF-.beta.2 which are used to construct expression vectors capable of directing the high-level expression of mature, biologically active TGF-.beta.2, as well as precursor TGF-.beta.2 forms, in transfected Chinese Hamster Ovary cells (CHO cells) and transfected COS cells are described. CHO and COS transfectants secreting TGF-.beta.2 at high levels are also described. CHO cells transfected with a plasmid vector carrying the complete 414 amino acid simian TGF-.beta.2 precursor secrete approximately 5 .mu.g per ml culture media.

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