Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1986-12-29
1990-08-28
Mays, Thomas
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
4351723, 435194, 435235, 4352523, 435320, 536 27, 935 14, 935 29, 935 31, 935 43, C12P 1934, C12N 1500, C12N 700, C12N 912
Patent
active
049524968
ABSTRACT:
This application describes a means to clone a functional gene for bacteriophage T7 RNA polymerase. Active T7 RNA polymerase is produced from the cloned gene, and a plasmid has been constructed that can produce the active enzyme in large amounts. T7 RNA polymerase transcribes DNA very efficiently and is highly selective for a relatively long promoter sequence. This enzyme is useful for synthesizing large amounts of RNA in vivo or in vitro, and is capable of producing a single RNA selectively from a complex mixture of DNAs. The procedure used to obtain a clone of the T7 RNA polymerase gene can be applied to other T7-like phages to obtain clones that produce RNA polymerases having different promoter specificities, different bacterial hosts, or other desirable properties. T7 RNA polymerase is also used in a system for selective, high-level synthesis of RNAs and proteins in suitable host cells.
REFERENCES:
Green et al. (1983) Cell vol. 32, pp. 681-694.
Studier et al. (1983) Cold Spring Harbor Symp. Quant. Biol. XLVII: 999-1007.
Stahl et al. (1981) J. Mol. Biol., vol. 148, pp. 481-485.
Calos (1978) Nature vol. 274, pp. 762-765.
Maniatis et al. (1982) Cold Spring Harbor Labs., pp. 413-417 in Molecular Cloning: Aldercolor Manual.
McAllister et al. (1981) J. Mol. Biol. 153, pp. 527-544.
Studier et al. (1981) J. Mol. Biol. 153, pp. 503-525.
Morris, et al., Gene, vol. 41, pp. 193-200 (1986).
Simon, et al., J. Mol. Biol., vol. 79, pp. 249-265 (1973).
Hyman, et al., Virology, vol. 57, pp. 189-206 (1974).
Studier, et al., J. Virology, vol. 19, No. 1, pp. 136-145 (1976).
Hyman, et al., J. Mol. Biol., vol. 71, pp. 573-582 (1972).
Studier, et al., J. Mol. Biol., vol. 135, pp. 917-937 (1979).
Rosa, Cell, vol. 16, pp. 815-825 (1979).
Chamerlin, et al., Methods in Enzymbolgy, vol. 101, pp. 540-568 (1983).
Bailey, et al., Proc. Natl. Acad. Sci. U.S.A., vol. 80, pp. 2814-2818 (1983).
Studier, J. Mol. Biol., vol. 79, pp. 237-248 (1973).
Davanloo Parichehre
Dunn John J.
Moffatt Barbara A.
Rosenberg Alan H.
Studier F. William
Associated Universities Inc.
Bogosian Margaret C.
Mays Thomas
LandOfFree
Cloning and expression of the gene for bacteriophage T7 RNA poly does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Cloning and expression of the gene for bacteriophage T7 RNA poly, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Cloning and expression of the gene for bacteriophage T7 RNA poly will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1589328