Cloning and expression of the ApaLI restriction endonuclease

Details Cloning and expression of the ApaLI restriction endonuclease Cloning and expression of the ApaLI restriction endonuclease

1995-05-24

1997-04-01

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

4353201, 4352523, 435193, 536 232, C12N 922, C12N 1555

Patent

active

056164840

ABSTRACT:
The present invention relates to a method of cloning ApaLI methylase gene (apaLIM) and ApaLI endonuclease gene (apaLIR) from Acetobacter pasteurianus into E. coli by the methylase selection method and inverse PCR. The ApaLI methylase gene was cloned into pUC19 (3 ApaLI sites inserted) by the methylase selection method. Eight ApaLI-resistant clones were isolated and found to contain apaLIM gene. However, these clones are not stable such that sometimes overnight cultures were lysed or plasmid DNA was lost in the unlysed culture.

REFERENCES:
patent: 4833082 (1989-05-01), Yamada et al.
Kosykh, et al., Molec. Gen. Genet. 178:717-718 (1980).
Mann, et al., Gene, 3:97-112 (1978).
Walder, et al., Proc. Natl. Acad. Sci. USA, 78:1503-1507 (1981).
Bougueleret, et al., Nucl. Acids Res. 12:3659-3676 (1984).
Gingeras and Brooks, Proc. Natl. Acad. Sci. USA, 80:402-406 (1983).
Theriault and Roy, Gene, 19:355-359 (1982).
Blumenthal, et al., J. Bacteriology, 164:501-509 (1985).
Kiss, et al., Nucl. Acids Res. 13:6403-6421 (1985).
Szomolanyi, et al., Gene, 10:219-225 (1980).
Janulaitis, et al., Gene, 20:197-204 (1982).
Kiss and Baldauf, Gene, 21:111-119 (1983).
Walder, et al., J. Biol. Chem., 258:1235-1241 (1983).
Fomenkov, et al., Nucl. Acids Res., 22:2399-2403 (1994).
Raleigh and Wilson, Proc. Natl. Acad. Sci. USA 83:9070-9074 (1986).
Heitman and Model, J. Bact. 169:3243-3250 (1987).

Affiliated with

Also associated with

Rating

Cloning and expression of the ApaLI restriction endonuclease does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Cloning and expression of the ApaLI restriction endonuclease, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Cloning and expression of the ApaLI restriction endonuclease will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-538252