Cloning and expression of the 47-kilodalton antigen of treponema

Details Cloning and expression of the 47-kilodalton antigen of treponema Cloning and expression of the 47-kilodalton antigen of treponema

1986-09-30

1989-09-19

Hazel, Blondel

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435320, 435 68, 530350, 424 88, C12N 120, C12N 1500, C12R 1185

Patent

active

048681180

ABSTRACT:
Monoclonal antibodies directed against the 47 kDa major outer membrane surface immunogen of virulent Treponema pallidum were used to select E. coli recombinant clones expressing the 47 kDa immunogen. The phenotype of the clones was dependent on the presence of recombinant plasmid in the host cell. Southern hybridization revealed that

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