Cloning and expression of T5 DNA polymerase reduced in 3'-to-5'

Details Cloning and expression of T5 DNA polymerase reduced in 3'-to-5' Cloning and expression of T5 DNA polymerase reduced in 3'-to-5'

1996-07-29

1998-02-10

Walsh, Stephen

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

435196, 4352523, 43525411, 4353201, 435325, 536 232, 536 241, C12N 1522, C12N 121, C12N 912, C12N 916

Patent

active

057168199

ABSTRACT:
The present invention discloses a recombinant DNA molecule having a structural gene encoding a processive, thioredoxin-independent DNA polymerase that is substantially reduced in processive 3'-to-5' DNA exonuclease activity, a promoter, and an origin of replication. A method for producing this enzyme is also disclosed, as is the protein produced by this process. This invention is exemplified by expression of exonuclease.sup.- T5 DNA polymerase in E. coli.

REFERENCES:
patent: 4394443 (1983-07-01), Weissman et al.
patent: 4689406 (1987-08-01), Banks et al.
patent: 4766066 (1988-08-01), Kuhstoss et al.
patent: 4795699 (1989-01-01), Tabor et al.
patent: 4889818 (1989-12-01), Gelfand et al.
patent: 5047342 (1991-09-01), Chatterjee
patent: 5102802 (1992-04-01), McAllister
patent: 5108892 (1992-04-01), Burke et al.
patent: 5270179 (1993-12-01), Chatterjee
patent: 5541099 (1996-07-01), Chatterjee
Bernad, A. et al., "A Conserved 3'.fwdarw.5' Exonuclease Active Site in Prokaryotic and Eukaryotic DNA Polymerases," Cell 59:219-228 (Oct. 1989).
Boswell, D. R., and A. M. Lesk, "Sequence comparison and alignment: the measurement and interpretation of sequence similarity," in: Computational Molecular Biology: Sources and Methods for Sequence Analysis, A. M. Lesk, Ed., Oxford University Press, Oxford, pp.161-163 (Feb. 1989).
Chatterjee, D. K. et al., "Cloning and overexpression of the gene encoding bacteriophage T5 DNA polymerase," Gene 97:13-19 (1991).
Creighton, T. E., in: Proteins: Structures and Molecular Properties, Second Edition, W. H. Freeman and Co., New York, N. Y., pp. 93-95 (1993).
Das, S. K. et al., "Effect of Mg.CTP.sup.2- and Mg.GTP.sup.2- on the 3'.fwdarw.5' Exonuclease Activity of T5-Induced DNA Polymerase," J. Carbohydrates, Nucleosides, Nucleotides 5(5):457-467 (1978).
Das, S. K. et al., "Effect of magnesium CTP(2-) and magnesium GTP(2-) on the 3'.fwdarw.5' exonuclease activity of T5-induced DNA polymerase," Chem. Abstr. 91:194, Abstract No. 1841r (1979).
Das, S. K. and R. K. Fujimura, "Mechanism of T5-induced DNA Polymerase: Replication of Short Primer Templates," J. Biol. Chem. 252(23):8700-8707 (1977).
Das, S.K. and R. K. Fujimura, "Mechanism of Primer-Template-dependent Conversion of dNTP.fwdarw.dNMP by T5 DNA Polymerase," J. Biol. Chem. 255(15):7149-7154 (1980).
Das, S. K. and R. K. Fujimura, "Processiveness of DNA Polymerases: A Comparative Study Using a Simple Procedure," J. Biol. Chem. 254(4):1227-1232 (1979).
Donlin, M. J. et al., "Kinetic Partitioning between the Exonuclease and Polymerase Sites in DNA Error Correction," Biochem. 30:538-546 (1991).
Fujimura, R. K. and B. C. Roop, "Characterization of DNA Polymerase Induced by Bacteriophage T5 with DNA Containing Single Strand Breaks," J. Biol. Chem. 251(7):2168-2175 (1976).
Fujimura, R. K. et al., "Physical Locus of the DNA Polymerase Gene and Genetic Maps of Bacteriophage T5 Mutants," J. Virol. 53(2):495-500 (1985).
Johnson, K. A. et al., "Elementary Steps of DNA Polymerization," DNA-Protein Interactions: Eighth Summer Symposium in Molecular Biology:44, 60-62 (Jul. 1989).
Joyce, C. M. and T. A. Steitz, "DNA polymerase I: from crystal structure to function via genetics", Trends Biochem. Sci. 12:288-292 (1987).
Kozak, M., "Comparison of Initiation of Protein Synthesis in Procaryotes, Eucaryotes, and Organelles," Microbiol. Rev. 47(1):1-45 (1983).
Leavitt, M. C. and J. Ito, "T5 DNA polymerase: Structural-functional relationships to other DNA polymerases," Proc. Natl. Acad. Sci. USA 86:4465-4469 (Jun. 1989).
Lewin, R., "When Does Homology Mean Something Else?" Science 237:1570 (1987).
Lin, T.-C. et al., "Cloning and expression of T4 DNA polymerase," Proc. Natl. Acad. Sci. USA 84:7000-7004 (1987).
Minkley, E. G. et al., "Escherichia coli DNA Polymerase I: Construction of a polA Plasmid for Amplification and an Improved Purification Scheme," J. Biol. Chem. 259(16):10386-10392 (1984).
Patel, S. S. et al., "Pre-Steady-State Kinetic Analysis of Processive DNA Replication Including Complete Characterization of an Exonuclease-Deficient Mutant," Biochem. 30:511-525 (1991).
Reeck, G. R. et al., "`Homology` in Proteins and Nucleic Acids: A Terminology Muddle and a Way out of It," Cell 50:667 (1987).
Reha-Krantz, L. J. et al., "DNA polymerization in the absence of exonucleolytic proofreading: In vivo and in vitro studies," Proc. Natl. Acad. Sci. USA 88:2417-2421 (1991).
Remaut, E. et al., "Plasmid vectors for high-efficiency expression controlled by the P.sub.L promoter of coliphage lambda," Gene 15:81-93 (1981).
Rhoades, M., "New Physical Map of Bacteriophage T5 DNA," J. Virol. 43(2):566-573 (1982).
Shibui, T. et al., "A New Hybrid Promoter and Its Expression Vector in Escherichia coli," Agric. Biol. Chem 52(4):983-988 (Apr. 1988).
Spicer, E. K. et al., "Primary Structure of T4 DNA Polymerase: Evolutionary Relatedness to Eucaryotic and Other Procaryotic DNA Polymerases," J. Biol. Chem. 263(16):7478-7486 (Jun. 1988).
Stark, M. J. R., "Multicopy expression vectors carrying the lac repressor gene for regulated high-level expression of genes in Escherichia coli," Gene 51:255-267 (1987).
Stueber, D. et al., "A novel in vitro transcription-translation system: accurate and efficient synthesis of single proteins from cloned DNA sequences," EMBO J. 3(13):3143-3148 (1984).
Tabor, S., and C. C. Richardson, "Selective Inactivation of the Exonuclease Activity of Bacteriophage T7 DNA Polymerase by in Vitro Mutagenesis," J. Biol. Chem. 264(11):6447-6458 (Apr. 1989).
Watson, J. D. et al., "Recombination at the Molecular Level," in: Molecular Biology of the Gene Fourth Edition, The Benjamin/Cummings Publishing Company, Inc., Menlo Park, CA, pp. 313-338 (1987).
Wong, I. et al., "An Induced-Fit Kinetic Mechanism for DNA Replication Fidelity: Direct Measurement by Single-Turnover Kinetics," Biochem. 30:526-537 (1991).

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