Cloning and expression of T5 DNA polymerase reduced in 3'- to-5'

Details Cloning and expression of T5 DNA polymerase reduced in 3'- to-5' Cloning and expression of T5 DNA polymerase reduced in 3'- to-5'

1992-01-28

1993-12-14

Low, Christopher S. F.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 698, 435 697, 435193, 435191, 4351723, 4353201, 4352523, 43525233, 435194, 536 231, 536 232, 536 2372, 536 241, 536 242, 536 234, 935 6, 935 14, 935 38, 935 72, C12P 2102, C12N 912

Patent

active

052701794

ABSTRACT:
The present invention discloses a recombinant DNA molecule having a full length or a truncated T5 DNA polymerase structural gene, each encoding a processive, thioredoxin-independent DNA polymerase. The DNA polymerase of the invention may have 3'-to-5'exonuclease activity or may be substantially reduced in processive 3'-to-5'DNA exonuclease activity. A method for producing these enzymes is also disclosed, as is the proteins produced by this process. The present invention is also directed to a leader sequence important for expression of soluble T5 DNA polymerase protein.

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