Cloning and expression of T5 DNA polymerase

Details Cloning and expression of T5 DNA polymerase Cloning and expression of T5 DNA polymerase

1989-08-10

1991-09-10

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

435239, 4352523, 43525233, 4353201, 4351723, 536 26, 536 27, 536 28, 536 29, 530826, C12N 912, C12N 120, C07H 1512, C07H 1906

Patent

active

050473425

ABSTRACT:
The present invention discloses a recombinant DNA molecule having a structural gene encoding a processive, thioredoxin-independent DNA polymerase, a promoter, and an origin of replication. The protein may also have a processive 3'-to-5' DNA exonuclease activity. A method for producing this enzyme is also disclosed, as is the protein produced by this process. This invention is exemplified by expression of T5 DNA polymerase in E. coli.

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