Cloning and expression of T4 DNA polymerase

Details Cloning and expression of T4 DNA polymerase Cloning and expression of T4 DNA polymerase

1986-09-18

1990-06-19

Teskin, Robin L.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435193, 435194, 435320, 4352523, 43525233, 536 27, 935 29, 935 41, 935 56, 935 73, C12N 1500, C12N 120

Patent

active

049353616

ABSTRACT:
The DNA sequence encoding T4 DNA polymerase (gene 43 of T4 phase) has been successfully cloned, thereby providing a convenient source of this enzyme. The method involves the production of a gene 43 NH.sub.2 -terminus fragment absent its promoter. The latter is ligated to a fragment containing the remaining COOH-terminus sequence but lacking base pairs beyond this terminus which heretofore caused destabilization effects in cloning. The full sequence of the DNA sequence has been elucidated. Corresponding cloning methods are disclosed.

REFERENCES:
patent: 4767708 (1988-08-01), Minkley et al.
Miller, R. C. et al., Regulation of the Synthesis of the T4 DNA Polymerase (Gene 43); Virology 110, pp. 98-112 (1981).
Holland, M. J. et al., Isolation and Characterization of a Generic Coding for G-3P-Dehydrogenase from S. cerevie; J. Biol. chem. 254 (12), pp. 5466-5479 (1979).
Morris, C. F. et al., J. Biol. Chem. 254, 6787-6796 (1979), p. 2, lines 24-28.
Wilson, G. G. et al., Mol. Gen. Genet. 156, 203-214 (1977): p. 2, line 28 to p. 3, line 1.

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