Cloning and expression of phosopholipase C genes

Details Cloning and expression of phosopholipase C genes Cloning and expression of phosopholipase C genes

1987-12-15

1991-04-02

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

435195, 4352401, 4352523, 43525233, 4353201, 536 27, C12N 900, C12N 914

Patent

active

050046926

ABSTRACT:
Improved means for producing Clostridium Phospholipase C (PL) polypeptides based on the cloning and expression of recombinant DNA segments containing Clostridium PLC genes and fragments. The DNA segments are operably linked to host specific expression control sequences for exogenous production of Clostridium PLC, or fragments thereof, substantially free from naturally-associated Clostridium gene products.

REFERENCES:
patent: 4140579 (1979-02-01), Moncla
patent: 4140754 (1979-02-01), Iwasa
Microgenie.RTM. User Manual, Beckman Instruments, Dec. 1988.
Pritchard, A. E. and Vasil, M. L., 1986, J. Bact., 167:291-298.
Krug, E. and Kent, C., 1984, Arch. Biochem. Biophys., 231(20:400-410).
Yamakawa, Y. and Ohsaka, A., 1977, J. Biochem., 81:115-126.
Kikutani, H. et al., 1986, Cell, 47:657-665, Dec. 5, 1986.

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