Cloning and expression of ligninases

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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4351721, 4353201, 536 231, 536 232, 536 237, C07H 2104, C12N 1531, C12N 1552, C12N 1581

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056164734

ABSTRACT:
The gene encoding the ligninase rLDM.TM. 6 has been cloned and expressed. The ligninases produced via the processes disclosed herein are useful to degrade or modify lignin in pulp operations.

REFERENCES:
Tren et al. "Lignin-degrading Enzyme from Phanerochaete chrysosporium: Purfication, Characterization and Catalytic Properties of a a unique H.sub.2 O.sub.2 -Requiring Oxygenase", PNAS, vol. 81, pp. 2280-2284, 1984.
Zhang et al. "Identification of a CDNA Clones for Ligninase from Phanerochaete chrysosporium Using Synthetic Oligonucleotide Probes", Biochem. Biophys. Res. Comm., vol. 137, No. 2, pp. 649-656, Jun. 13, 1986.
Alberts et al. "Molecular Biology of the Cell", Garland Publishing Co., Inc., New York (1983), pp. 184-193.
Watson et al. "Recombinant DNA: A Short Course", W.H. Freeman and Co., New York (1983) pp. 50-90.
Haylock et al. "Molecular Genetics of Phanerochaete chrysosporium" Confernece of Lignocellulose Biodegradtion, Abstract, 1984, pp. 365-366.
R. Haylock, et al. "Isolation of mRNA from P. chrysosporium and its In Vitro Translation". J. Microbiol. Methods, 1985, 4(3-4), pp. 155-162.
U. Raeder, etal. "Rapid Preparation of DNA from Filamentous Fungi", Letters in Applied Microbiol. 1985, 1(1), pp. 17-20.
M. Tien, et al. "Cloning and Sequencing of acDNA for a Ligninase from P. chrysosporium", Nature. vol. 326, 2 Apr. 1987, pp. 520-523.
Corrigendum for the above (Reference AU), Nature vol. 328, 20 Aug. 1987, p. 742.

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