Cloning and expression of host-protective immunogens of IBDV

Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Fusion protein or fusion polypeptide

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4242041, 435 693, 435 697, 530350, 530395, 530403, A61K 3912, C07K 1401

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active

060541261

ABSTRACT:
This invention relates to the cloning and characterization of the infectious bursal disease virus (IBDV) genome, to the identification of cloned genes for host-protective antigens of IBDV, to the expression of cDNA inserts encoding the whole or part of host-protective antigens of IBDV in E.coli or other host cells, and to the use of the expressed antigens in the production of virus neutralizing antibodies in chickens. The invention also relates to the production of an effective sub-unit vaccine against IBDV utilizing the expressed antigens, as well as to the use of the expressed antigens in diagnostic tests, assays and the like.

REFERENCES:
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Hudson, L. et al. Practical Immunology (1980). pp. 8-9. Blackwell Sci. Publications.
Jackwood, D.J. et al. Avian Dis. 28:990-1006 (1984) Nucleic Acid and Structural Proteins of I.B.D.V. Isolaters Belonging to Serotypes I and II.
MacReadie I.G. et al. Vaccine 8:549-552 (1990) "Passive Protection Against I.B.D.V. by Viral VP2 Expressed in Yeast.".
Becht, H. Curr. Top. Microbiol. Immunol. 90:107-121 (1980), "Infectious Bursal Virus Disease".
Dobos, P. J. Virology 32:1046-50 (1979), "Peptide Map Comparison of the Proteins of I.B.D.V.".
Fahey, K.J. et al. J. Gen. Virol. 66:1479-88 (1985) "Characterization by Western Blotting of the Immunogens of I.B.D.V.".
Fahey, K.J. et al. J. Gen. Virol. 66:2693-2702 (1985) "Antibody to the 32K Structural Protein of I.B.D.V. Neutralizes Viral Infectivity in Vitro and Confers Protection on Young Chickens.".
Muller, et al. J. Virology 44:384-92 (1982) Biosynthesis of Virus-Specific Protein in Cells Infected with I.B.D.V. and their Significance as Structural Elements for Infectious Virus and Incomplete Particles.

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