Cloning and expression of complementary DNAs for multiple member

Details Cloning and expression of complementary DNAs for multiple member Cloning and expression of complementary DNAs for multiple member

1994-02-22

1998-07-28

Jacobson, Dian C.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

435 72, 435 691, 4352402, 4352523, 4352551, 4353201, 514 2, 514 12, 536 221, 536 231, 536 232, 536 235, C12N 902, C12N 120, C12P 2106, C07H 1900

Patent

active

057861919

ABSTRACT:
The invention provides two novel members of the cytochrome P450 2C subfamily of enzymes, designated 2C18 and 2C19. DNA segments encoding these enzymes are also provided. The 2C19 polypeptide represents the principal human determinant of human S-mephenytoin 4'-hydroxylase activity. The invention also provides methods of identifying drugs metabolized by S-mephenytoin 4'-hydroxylase activity. Drugs shown to be metabolized by this activity should in general not be administered to individuals having, or belong to an ethnic group at risk of, a polymorphic deficiency in S-mephenytoin 4'-hydroxylase activity.

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