Cloning and expression of autogenes encoding RNA polymerases of

Details Cloning and expression of autogenes encoding RNA polymerases of Cloning and expression of autogenes encoding RNA polymerases of

1996-11-08

1998-11-03

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435194, 4352523, 4353201, C12P 2102, C12N 912, C12N 121, C12N 1563

Patent

active

058306943

ABSTRACT:
This invention relates to the cloning and expression of autogenes encoding RNA polymerases of T7 and T7-like bacteriophages, in which the RNA polymerase gene is transcribed from a promoter which is recognized by the encoded RNA polymerase. Cloning of T7 autogenes was achieved by reducing the activity of the RNA polymerase sufficiently to permit host cell growth. T7 RNA polymerase activity was controlled by combining two independent methods: lac-repression of the recombinant lac operator-T7 promoter in the autogene and inhibition of the polymerase by T7 lysozyme. Expression systems for producing the RNA polymerases of T7 and other T7-like bacteriophages, and expression systems for producing selected gene products are described, as well as other related materials and methods.

REFERENCES:
Dunn, J.J. et al. (1983) "Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements" J. Mol. Biol. 166:477-535.
Dubendorff, J.W. et al. (May 1991) "Creation of a T autogene" J. Mol Biol. 219:61-68.

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