Cloning and expression of acetyl xylan esterases from fungal ori

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

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435691, 435712, 4352523, 43525231, 4353201, 4351723, 536 232, 935 14, 935 29, 935 56, 426656, 426635, C12N 918, C12N 1555, A23K 100

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054260434

ABSTRACT:
Methods and DNA constructs are provided for the expression of a fungal acetyl xylan esterase gene in microbial hosts. A purified fungal acetyl xylan esterase is obtained which is suited for the use as an accessory enzyme in the degradation of acetylated xylans.

REFERENCES:
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S. L. Berger. et al. "Guide to Molecular Cloning Techniques." Meth. in Enzymol. vol. 152 pp. 393-399, 415-447, 661-704 (1987).
M. P. Deutscher "Guide to Protein Purification" Meth. in Enzymol. vol. 182 pp. 602-613, 738-751 (1990).
A. Belyansky et al. "PCR-based cDNA library construction . . . " Nuc. Acids Res. 17(8) 2919-2932 (Apr. 1989).
Sundberg et al., Biotechnology & Applied Biochemistry (1991) 13(1):1-11.
Biely et al., Canadian Journal of Microbiology (1988) 34(6):767-772.
Poutanen et al., Applied Microbiology and Biotechnology (1990) 33:506-510.
Biley et al., FEBS Letters (1985) 186(1):80-84.
Grohmann et al., Applied Biochemistry and Biotechnology (1989) 20/21:45-61.

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