Cloning and expression of a gene encoding bryodin 1 from Bryonia

Details Cloning and expression of a gene encoding bryodin 1 from Bryonia Cloning and expression of a gene encoding bryodin 1 from Bryonia

1994-05-17

1996-07-30

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

4353201, 435199, 536 232, 536 236, 530350, C12N 120, C12N 1563, C12N 922, C07H 2104, C07K 1400

Patent

active

055411100

ABSTRACT:
The molecular cloning and expression of biologically active ribosome-inactivating protein bryodin 1 are described. A complete amino acid and oligonucleotide sequence encoding bryodin 1 are also described. Further, plasmids, expression vectors comprising a nucleotide sequence encoding bryodin 1 and transformed host cells are described. Isolation and characterization of the nucleotide sequence for bryodin 1 enables the recombinant production of large amount of bryodin 1 for use in vitro or in vivo directly or as ligand/toxin conjugates or fusion proteins. These compositions can be used to selectively kill undesired cells such as cancer cells, infected cells, bacteria.

REFERENCES:
C. Z. Amorim et al., "Screening of the Antimalarial Activity of Plants of the Cucurbitaceae Family," Mem. Inst. Oswaldo Cruz 86:177-180, 1991.
Y. Endo et al., "RNA N-Glycosidase Activity of Ricin A-chain," J. Biol. Chem. 262:8128-8130, 1987.
P.-C. Montecucchi et al., "N-terminal sequence of some ribosome-inactivating proteins," Int. J. Peptide Protein Res. 33:263-267, 1989.
T. B. Ng et al., "Proteins with abortifacient, ribosome in activating, immunomodulatory, antitumor and anti-AIDS activities from Cucurbitaceae plants," Gen. Pharmac. 23:575-590, 1992.
F. Stirpe et al., "Bryodin, a ribosome-inactivating protein from the roots of Bryonia dioica L. (white bryony)," Biochem. J. 240:659-665, 1986.
F. Stirpe et al., "Modification of ribosomal RNA by ribosome-inactivating proteins from plants," Nucleic Acids Research 16:1349-1357, 1988.

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