Cloning and expressing XhoII restriction endonuclease and M.XhoI

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease

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435199, 4353201, 435252, 536 232, C12N 910, C12N 922, C12N 1554, C12N 1559

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active

053044800

ABSTRACT:
The present invention is directed to recombinant hosts which contain and express XhoI and XhoII Type II restriction endonuclease and/or M.XhoI and M.XhoII modification methylase genes. The present invention is also directed to vectors or DNA molecules which contain these genes, and to methods of producing these enzymes. One source of these enzymes is Xanthomonas campestris pv. holcicola, although other microorganisms may be used to isolate the restriction endonuclease isoschizomers and modification methylase isoschizomers of this invention.

REFERENCES:
patent: 4983542 (1991-01-01), Van Cott et al.
patent: 4996151 (1991-02-01), Brooks et al.
patent: 5002882 (1991-03-01), Lunnen et al.
patent: 5082784 (1992-01-01), Chatterjee et al.
patent: 5147800 (1992-09-01), Hammond et al.
patent: 5179015 (1993-01-01), Wilson et al.
patent: 5200333 (1993-04-01), Wilson
patent: 5231021 (1993-07-01), Chatterjee
Kessler, C. et al (1990) Gene 92, 1, 28.
Lunnen, K. P. et al, (1988) Gene 74, 25-32.
Wilson, G. G. (1988) Gene 74, 281-289.
Wilson, G. G. (1988) TIG 4(11), 314-318.
Brooks et al., Cloning the BamHI restriction modification system, Nucleic Acids Research 17(3):979-997 (1989).
Darzins et al., Cloning of Genes Controlling Alginate Biosynthesis from a Mucoid Cystic Fibrosis Isolate of Pseudomonas aeruginosa, Journal of Bacteriology 159(1):9-18 (Jul. 1984).
Gingeras et al., A New Specific Endonuclease Present in Xanthomonas holcicola, Xanthomonas papavericola and Brevibacterium luteum, J. Mol. Biol. 118:113-122 (1978).
Hammond et al., Cloning the KpnI restriction-modification system in Escherichia coli, Gene 97:97-102 (1991).
Howard et al., Cloning the DdeI restriction-modification system using a two-step method, Nucleic Acids Research 14(20:7939-7951 (1986).
Janulaitis et al., Cloning of the modification methylase gene of Bacillus centrosporus in Escherichia coli, Gene 20:197-204 (1982).
Kessler et al., Specificity of restriction endonucleases and DNA modification methyltransferases-a review (Edition 3), Gene 92:28 and 80 (1990).
Kiss et al., Molecular cloning and expression in Escherichia coli of two modification methylase genes of Bacillus subtilis, Gene 21:111-119 (1983).
Mann et al., Cloning of Restriction and Modification Genes in E. coli: The HhaII System From Haemophilus haemolyticus, Gene 3:97-112 (1978).
Piekarowicz et al., A new method for the rapid identification of genes encoding restriction and modification enzymes, Nucleic Acids Research 19(8):1831-1835 (1991).
Roberts, R. J., Restriction enzymes and their isoschizomers, Nucleic Acids Research 17 (Suppl):r347-r387 (1989).
Stratagene Catalog p. 114 (1989).
Szomolanyi et al., Cloning the modification methylase gene of Bacillus sphaericus R in Escherichia coli, Gene 10:219-225 (1980).
Walder et al., Cloning of the MspI Modification Enzume, The Site of Modification and Its Effects on Cleavage by MspI and HpaII, The Journal of Biological Chemistry 258(2):1235-1241 (Jan. 25, 1983).
Wilson, G. G., Organization of restriction-modification systems, Nucleic Acids Research 19(10):2539-2566 (1991).

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