Cloning and expressing restriction endonucleases and modificatio

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease

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435199, 4353201, 43525234, 536 232, C12N 910, C12N 922, C12N 1554, C12N 1555

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052310213

ABSTRACT:
The present invention is directed to recombinant hosts which contain and express XhoI and XhoII Type II restriction endonuclease and/or M.XhoI and M.XhoII modification methylase genes. The present invention is also directed to vectors or DNA molecules which contain these genes, and to methods of producing these enzymes. One source of these enzymes is Xanthomonas campestris pv. holcicola, although other microorganisms may be used to isolate the restriction endonuclease isoschizomers and modification methylase isoschizomers of this invention.

REFERENCES:
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