Cloning and developmental expression of pea ribulose-1,5-bisphos

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease

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536 232, C12N 910, C07H 2104

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058663940

ABSTRACT:
The gene sequence for ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) large subunit (LS) .sup..epsilon. N-methyltransferase (protein methylase III or Rubisco LSMT) is disclosed. This enzyme catalyzes methylation of the .epsilon.-amine of lysine-14 in the large subunit of Rubisco. In addition, a full-length cDNA clone for Rubisco LSMT is disclosed. Transgenic plants and methods of producing same which (1) have the Rubisco LSMT gene inserted into the DNA, and (2) have the Rubisco LSMT gene product or the action of the gene product deleted from the DNA are also provided. Further, methods of using the gene to selectively deliver desired agents to a plant are also disclosed.

REFERENCES:
patent: 5422108 (1995-06-01), Mirkov et al.
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Klein et al., "Cloning and Development Expression of PEA Ribulose-1,5-bisphosphate carboxylase/oxygenase Large Subunit N-Methyltransferase", Plant Molecular Biology 27:249-261 (1995).
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