Cloning and characterisation of novel mammalian peptidase

Details Cloning and characterisation of novel mammalian peptidase Cloning and characterisation of novel mammalian peptidase

2007-05-15

2007-05-15

Nashed, Nashaat T. (Department: 1656)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

C435S069100, C435S252300, C435S320100, C536S023200

Reexamination Certificate

active

09743647

ABSTRACT:
There is disclosed a cDNA molecule encoding human NAALAD-ases designated herein as L, II and IV and having the amino acid sequences illustrated in FIGS.1, 3, 4and5. The NAALAD-ases themselves also form part of the invention. Further provided are expression vectors incorporating the cDNA molecules suitable for transforming a cell, tissue or organism.

REFERENCES:
patent: WO 96/15258 (1996-05-01), None
Carter, R.E., et al., 1996,: “Prostate-specific membrane antigen is a hydrolase with substrate and pharmacologic characteristics of a neuropeptidase”, Proceedings of the National Academy of Sciences, USA, vol. 93, pp. 749-753.
Shneider, B.L., et al. 1997, “Cloning and characterization of a novel peptidase from rat and human ileum”, The Journal of Biological Chemistry, vol. 272, No. 49, pp. 31006-31015.
Luthi-Carter, R. et al., 1998, “Isolation and characterization of a rat brain cDNA encoding glutamante carboxypeptidase II,” Proceedings of the National Academy of Sciences, USA, vol. 95, pp. 3215-3220.
Pangalos, H.N., et al., 1999, “Isolation and expression of novel human glutamate carboxyqeptidases with N-acetylated alpha-lInked acidic dipeptldase peptidase IV activity”, The Journal of Biological Chemistry, vol. 274, No. 13, pp. 8470-8483.
Seffernick, J.L., et al., 2001, “Melamine deaminase and atrazine chlorohydrolase: 98 percent identical but functionally different”, Journal of Bacteriology, vol. 183, No. 8, pp. 2405-2410.

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