Cloned SstI/SacI restriction-modification system

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435193, 4352523, 43525233, 4353201, 536 232, C12N 922, C12N 1555, C12N 1570

Patent

active

057598398

ABSTRACT:
The present invention discloses the cloning and expression in Escherichia coli of the SstI/SacI restriction-modification system. The SstI methylase gene was cloned by methylase selection (the "Hungarian Trick" and the orientation and boundaries of the gene were established. The level of methylation of the host was improved significantly by expressing the SstI methylase from the strong RPSL promoter. Using the SstI methylase clone as a probe, a chromosomal map was generated in the area of the gene for the SstI methylase. DNA downstream from the methylase was cloned and found to contain the gene for the SstI endonuclease. The orientation of the SstI endonuclease was established and a strong promoter was placed close to the SstI endonuclease gene by generating nested deletions. By these methods, an E. coli strain was constructed which produced high levels of SstI endonuclease. The SacI locus in the S. achromogenes chromosome was found to be identical to the SstI locus in S. stanford in all measured respects.

REFERENCES:
patent: 5288696 (1994-02-01), Guthrie et al.
patent: 5334526 (1994-08-01), Smith et al.
patent: 5532153 (1996-07-01), Xu et al.
patent: 5534428 (1996-07-01), Longo et al.
Goff, S.P., et. al. (1978) Gene 13, 347-352.
Zhuravleva, L.I., et. al.(1987)Prikl. Biochim. Mikrobilo. 23, 208-215.
Blumenthal, R. M. et al., "Cloning of a Restriction-Modification System from Proteus vulgaris and Its Use in Analyzing a Methylase-Sensitive Phenotype in Escherichia coli," J. Bacteriol. 164(2):501-509 (1985).
Bougueleret, L. et al., "Characterization of the genes coding for the Eco RV restriction and modification system of Escherichia coli," Nucleic Acids Res. 12(8):3659-3676 (1984).
Brooks, J. E. et al., "Cloning the BamHI restriction modification system," Nucleic Acids Res. 17(3):979-997 (1989).
Gingeras, T. R. and J. E. Brooks, "Cloned restriction/modification system from Pseudomonas aeruginosa," Proc. Natl. Acad. Sci. USA 80:402-406 (1983).
Goff, S. P. and A. Rambach, "SstI: A Restriction Endonuclease from Streptomyces sp. stanford," Gene 3:347-352 (1978).
Greene, P. J. et al., "Sequence Analysis of the DNA Encoding the Eco RI Endonuclease and Methylase," J. Biol. Chem. 256(5):2143-2153 (1981).
Hammond, A. W. et al., "Cloning the KpnI restriction-modification system in Escherichia coli," Gene 97:97-102 (1990).
Heitman, J. and P. Model, "Site-Specific Methylases Induce the SOS DNA Repair Response in Escherichia coli," J. Bacteriol. 169(7):3243-3250 (1987).
Howard, K. A. et al., "Cloning the DdeI restriction-modification system using a two-step method," Nucleic Acids Res. 14(20):7939-7951 (1986).
Ikemura, T. et al., "Isolation and Characterization of Stable Hybrid mRNA Molecules Transcribed from Ribosomal Protein Promoters in E. coli," Cell 18:895-903 (1979).
Janulaitas, A. et al., "Cloning of the modification methylase gene of Bacillus centrosporus in Escherichia coli," Gene 20:197-204 (1982).
Kiss, A. and F. Baldauf, "Molecular cloning and expression in Escherichia coli of two modification methylase genes of Bacillus subtilis ," Gene 21:111-119 (1983).
Kosykh, V. G. et al., "Molecular cloning of EcoRII Endonuclease and Methylase Genes," Molec. gen. Genet. 178:717-718 (1980).
Lisser, S. and H. Margalit, "Compilation of E. Coli mRNA promoter sequences," Nucleic Acids Res. 21(7):1507-1516 (Apr. 1993).
Mann, M. B. et al., "Cloning of Restriction and Modification Genes in E. Coli:The HhaII System from Haemophilus haemolyticus," Gene 3:97-112 (1978).
Newman, A. K. et al., "DNA Sequences of Structural Genes for Eco RI DNA Restriction and Modification Enzymes," J. Biol. Chem. 256(5):2131-2139 (1981).
Post, L. E. et al., "DNA Sequences of Promoter Regions for the str and spc Ribosomal Protein Operons in E. coli," Cell 15:215-229 (1978).
Raleigh, E. A. and G. Wilson, "Escherichia coli K-12 restricts DNA containing 5-methylcytosine," Proc. Natl. Acad. Sci. USA 83:9070-9074 (1986).
Reddy, P. et al., "Hyperexpression and purification of Escherichia coli adenylate cyclase using a vector designed for expression of lethal gene products," Nucleic Acids Res. 17(24):10473-10488 (1989).
Revel, H. R., "Restriction of Nonglucosylated T-even Bacteriophage: Properties of Permissive Mutants of Escherichia coli B and K12," Virol. 31:688-701 (1967).
Rodicio, M. R. and K. F. Chater, "Cloning and expression of the Sal I restriction-modification genes of Streptomyces albus G," Mol. Gen. Genet. 213:346-353 (1988).
Rosenberg, M. and D. Court, "Regulatory Sequences Involved in the Promotion and Termination of RNA Transcription," Ann. Rev. Genet. 13:319-353 (1979).
Szomolanyi, E. et al., "Cloning the modification methylase gene of Bacillus sphaericus R in Escherichia coli," Gene 10:219-225 (1980).
Miskliga, D., et. al. "Identification of an A-factor-dependent promoter in the streptomycin biosynthetic Gene cluster of Streptomyces griseus," Mol. Gen. Genet. 229:119-128 (1991).
Walder, R. Y. et al., "Cloning and Expression of the Pst I restriction-modification system in Escherichia coli," Proc. Natl. Acad. Sci. USA 78(3):1503-1507 (1981).
Walder, R. Y. et al., "Cloning of the MspI Modification Enzyme: The Site of Modification and Its Effects on Cleavage by MspI and HpaII," J. Biol. Chem. 258(2):1235-1241 (1983).
Wilson, G. G., "Cloned restriction-modification systems--a review," Gene 74:281-289 (1988).
Zhuravleva, L. I. et al., "Isolation and Purification of Restriction Endonuclease Sac I from Streptomyces achromogenes ATCC 12767," Prikl. Biokhim. Mikrobiol. 23(2):208-215 (1987).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Cloned SstI/SacI restriction-modification system does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Cloned SstI/SacI restriction-modification system, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Cloned SstI/SacI restriction-modification system will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1458691

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.