Cloned pullulanase

Details Cloned pullulanase Cloned pullulanase

1994-12-28

1996-05-07

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435 691, 4352523, 43525231, 43525233, 43525421, 4353201, 536 232, C12N 944, C12N 1556, C12N 1575, C12N 1581

Patent

active

055145760

ABSTRACT:
A method is disclosed for the expression of an active pullulanase enzyme in a microorganism host. In one aspect, a DNA construct contains a sequence encoding the pullulanase enzyme, except for the nucleotides necessary to encode the first two amino acids in mature pullulanase, and regulating sequences allowing expression of the coding sequence in a microorganism host. An advantageous DNA construct contains regulatory sequences permitting expression of the pullulanase in a yeast cell.

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