Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Patent
1993-11-04
1995-11-28
Schwartz, Richard A.
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
4352523, 43525231, 43525234, 43525235, 4352402, 4353201, 536 232, C12N 1555, C12N 121, C12N 510, C12N 1570
Patent
active
054707404
ABSTRACT:
The present invention discloses the cloning and expression in Escherichia coli of the NsiI restriction-modification system from Neisseria sicca, utilizing a two step protocol. Initial protection of E. coli host with methylase expressed on a plasmid was required to stabilize a compatible plasmid carrying the endonuclease gene on a single DNA fragment. A chromosomal map was generated localizing the genes for NsiI methylase and endonuclease. An E. coli strain was constructed which produced high levels of NsiI endonuclease.
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Longo Mary C.
Smith Michael D.
Life Technologies Inc.
Schmickel David B.
Schwartz Richard A.
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