Cloned Leuconostoc mesenteroides glucose-6-phosphate dehydrogena

Details Cloned Leuconostoc mesenteroides glucose-6-phosphate dehydrogena Cloned Leuconostoc mesenteroides glucose-6-phosphate dehydrogena

1990-10-12

1993-09-14

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

435 691, 4351723, 43525233, 4353201, 536 232, 536 243, 935 14, 935 8, C12N 904, C12N 1511, C12N 1553

Patent

active

052447960

ABSTRACT:
The Glc6PD genome of Leuconostoc mesenteroides is isolated. Oligonucleotides complementary to sequences upstream and downstream to the Glc6PD genome are prepared and used to amplify the genome by the polymerase chain reaction. The amplified gene is isolated and cloned into pUC19. The recombinant plasmid expresses the gene in E. coli.

REFERENCES:
patent: 4652639 (1987-03-01), Stubinsky
patent: 4686181 (1987-08-01), Dona
patent: 5137821 (1992-08-01), Sagai et al.
Levy, H. R., 1988, Biochemical Society Transactions, 17: 313-315.
Jaye, M., et al., 1983, Nucleic Acids Research, 11(8): 2325-2335.
Bhadbhade, M. M., et al., 1987, FEBS Letters, 211(2): 243-246.
Lathe, R., 1985, Journal of Molecular Biology 183: 1-12.
Product Description from Boehringer Mannheim Corp. Printed Nov. 1990-Glucose-6-Phosphate Dehydrogenase.
Murphy, Noel B. et al., "Expression of other Gene for NAD-Dependent Glucose-6-Phosphate Dehydrogenase from Leuconostoc Mesenteroides Cloned in Escherichia coli K-12". Journal of Bacteriology, vol. 169 No. 1, pp. 334-339, Jan. 1987.

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