Cloned leptospira outer membrane protein

Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Parasitic organism or component thereof or substance...

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530350, 530820, 530822, 4242651, A61K 39002

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058243216

ABSTRACT:
An antigenic preparation is provided which contains a 63 Kd outer membrane protein from Leptospira which can be used immunologically as a vaccine for leptospirosis caused by this organism. Also provided in the invention are polynucleotides encoding the protein and antibodies which bind the protein which are useful in the diagnosis of leptospirosis.

REFERENCES:
patent: 5679353 (1997-10-01), Hall et al.
Dunn et al., "Outer Surface Protein A (OspA) from the Lyme Disease Spirochete, Borrelia burgdorferi: High Level Expression and Purification of a Soluble Recombinant Form of OspA," Protein Expression and Purification, vol. 1, No. 2, (2pgs.).
Kida et al., "Immunological and Morphological Analysis of Sodium Dodecyl Sulfate Extract of Leptospira," Zbl. Bakt. HY6 I. ABT. Orig. A 236, pp. 328-335 (1976).
Nicholson, Vivian and Prescott, John, "Outer Membrane proteins of three pathogenic Leptospira species," Veterinary Microbiology, 36, pp. 123-138 (1993).
Zuerner et al., "Characterization of outer member and secreted proteins of Leptospira interrogans serovar pomona," Microbial Pathogenesis, 10:pp. 311-322, (1991).
Brown et al., "Protein and Antigen Profiles of Prevalent Serovars of Leptospira interrogans," Infection and Immunity, vol. 59, No. 5, pp. 1772-1777 (May, 1991).
Haake et al., "Molecular Cloning and Sequence Analaysis of the Gene Encoding OmpL 1, a Transmembrane Outer Membrane Protein of Pathogenic Leptospira spp.," Journal of Bacteriology, vol. 175, No. 13, pp. 4225-4234, (Jul., 1993).
Nunes-Edwards et al. "Identification and Characterization of the Protein Antigens of Leptospira interrogans serovar hardjo," Infection and Immunity, vol. 48, No. 2, pp. 492-497, (May, 1985).

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