Cloned high signal strength promoters

Details Cloned high signal strength promoters Cloned high signal strength promoters

1981-05-20

1985-01-22

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 29, 435 68, 4351723, 435 91, 536 27, 935 6, 935 36, 935 37, 935 41, 935 76, C12Q 168, C12Q 102, C12P 2100, C12P 1934, C12N 1500

Patent

active

044952805

ABSTRACT:
Method for preparing high signal strength promoters and terminators and DNA compositions employing such promoters and terminators. T5 phage is cleaved to provide for DNA sequences having intact promoters. These promoters are inserted into vectors separated from a balanced terminator by a gene of interest and the terminator is desirably followed by a marker allowing for selection of transformants. High efficiencies in transcription of DNA can be achieved with the highly active T5 promoters. The promoters and terminators are used in hybrid DNA for efficient expression of structural genes and transcription to provide RNA sequences.

REFERENCES:
Stuber et al., PNAS, 78(1), 167-171, (Jan. 1981).
Casadaban et al., J. Mol. Biol., 138, 179-207, (1980).
West et al., Gene, 9, 175-193, (1980).
Gabain et al., PNAS, 76(1), 189-193, (1979).
Stuber et al., Molec. Gen. Genet., 166, 141-149, (1978).
Stuber et al., PNAS USA, 78(1), 167-171, (1981).

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