Cloned genes encoding reverse transcriptase lacking RNase H acti

Details Cloned genes encoding reverse transcriptase lacking RNase H acti Cloned genes encoding reverse transcriptase lacking RNase H acti

1991-03-18

1993-09-14

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

435697, 4351723, 43525233, 4353201, 536 234, C12N 912, C12N 1500, C12N 1554, C12N 1570

Patent

active

052447979

ABSTRACT:
The invention relates to a gene which encodes reverse transcriptase having DNA polymerase activity and substantially no RNase H activity. The invention also relates to vectors containing the gene and hosts transformed with the vectors of the invention. The invention also relates to a method of producing reverse transcriptase having DNA polymerase activity and substantially no RNase H activity by expressing the reverse transcriptase genes of the present invention in a host. The invention also relates to a method of producing cDNA from mRNA using the reverse transcriptase of the invention. The invention also relates to a kit for the preparation of cDNA from mRNA comprising the reverse transcriptase of the invention.

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