Cloned genes encoding reverse transcriptase lacking RNase H acti

Details Cloned genes encoding reverse transcriptase lacking RNase H acti Cloned genes encoding reverse transcriptase lacking RNase H acti

1992-01-24

1995-04-11

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

536 234, 536 232, 435194, 435 691, 435 697, 4352523, 4353201, 935 10, 935 14, 935 29, 935 36, 935 47, 935 66, 935 73, C12N 912, C12N 1500, C12N 1554, C12N 1570

Patent

active

054057760

ABSTRACT:
The invention relates to a gene which encodes reverse transcriptase having DNA polymerase activity and substantially no RNase H activity. The invention also relates to vectors containing the gene and hosts transformed with the vectors of the invention. The invention also relates to a method of producing reverse transcriptase having DNA polymerase activity and substantially no RNase H activity by expressing the reverse transcriptase genes of the present invention in a host. The invention also relates to a method of producing cDNA from mRNA using the reverse transcriptase of the invention. The invention also relates to a kit for the preparation of cDNA from mRNA comprising the reverse transcriptase of the invention.

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